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微囊化肝细胞移植对急性肝衰竭大鼠肝组织Smac及细胞色素c mRNA表达的影响

Smac/DIABLO and cytochrome c mRNA levels in liver tissue of rats with acute hepatic failure treated by microencapsulated hepatocytes

摘要:

目的 动态观察微囊化肝细胞腹腔移植对急性肝衰竭大鼠肝组织Smac/DIABLO及细胞色素c(cyt-e)mRNA表达的影响.方法 D-氨基半乳糖诱导大鼠急性肝衰竭模型,并设微囊化肝细胞移植组、裸肝细胞移植组和对照组.RT-PeR法测定大鼠肝脏凋亡指标Smac/DIABLO和cyt-cmRNA表达情况.样本比较采用重复测量的多元方差分析或单因素方差分析,组间比较采用t检验.结果 急性肝衰竭大鼠肝组织中Smac/DIABLO及cyt-c mRNA的表达较对照组明显增加(F值为4.345、14.821、47.565、42.178、62.961,P值均<0.05).裸肝细胞移植组和对照组肝组织中的Smac/DIABLO和cyt-c mRNA表达在48 h最高,而微囊化肝细胞移植组的峰值略微前移,在24 h最高.结论 Smac/DIABLO及cyt-c mRNA的表达随着急性肝衰竭的进程而波动,可以较好地判断肝细胞凋亡情况.

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abstracts:

Objective To investigate Smac/DIABLO and cytochrome c(cyt-c)mRNA levels in liver tissue of rats with acute hepatic failure treated by microencapsulated hepatocyte.Methods Acute hepatic failure were induced by intraperitoneal injection of D-galactosamine in rats.and the rats were treated with microencapsulated hepatocytes,free hepatocytes and physical saline(contr01),respectively.Smac/DIABLO and cyt-c mRNA in liver tissue was detected by RT-PCR and the mRNA expression levels among three groups were compared.Results Smac/DIABLO and cyt-c mRNA levels in liver tissues of rats with acute hepatic failure were higher than those of normal rata(F=4.345,14.821,47.565,42.178 and 62.961,P<0.05).The peak values of Smac/DIABLO and cyt-C mRNA expressions in free hepatocytes and control groups were at 48 h.while that in microencapsulated hepatoeytes group was at 24 h.Conclusion Smac/DIABLO and cyt-c mRNA expression is an indicator of apoptosis of hepatocytes.

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