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目的 探讨白藜芦醇对兔动脉粥样硬化斑块内细胞凋亡的作用. 方法成熟健康雄性新西兰白兔70只,适应性喂养10 d后随机分为5组:正常对照组给予普通饲料,病理对照组及白藜芦醇下预组给予高脂饲料,干预组同时每天分别给予4 mg/kg、8 rag/kg和16 mg/kg的白藜芦醇;苏木精-伊红染色观察病理组织学变化,脱氧核苷酸未端转移酶介导的脱氧三磷酸尿苷缺口末端标记法(TUNEI)榆测细胞凋亡. 结果高脂饲料喂养12周后,成功建立兔动脉粥样硬化模型.正常对照组血管内膜偶见少量的凋亡细胞,病理对照组有严重的动脉粥样硬化病理改变,粥样斑块内有大量的凋亡细胞.两组细胞凋亡指数分别为2.21±0.31和10.62±1.53(P<0.01);白藜芦醇干预均可抑制斑块内细胞的凋亡,且具有剂量依赖性.与病理对照组比较,低剂量组细胞凋亡指数为9.07±1.41(P<0.05),中、高剂量组分别为7.55±0.96和5.41±1.00(P<0.01). 结论白藜芦醇可抑制兔动脉粥样硬化斑块内的细胞凋亡.从而稳定斑块、抑制动脉粥样硬化病变进展.

Objective To investigate the effects of resveratrol on the apoptosis in atherosclerotic plaque of rabbit. Methods Seventy male New Zealand white rabbits were randomly divided into 5 groups: A group for normal control, B group for pathological control , C group for pathological control treated with low dosage resveratrol (4 rag · kg~(-1)·d~(-1)), D group for pathological control treated with middle dosage resveratrol (8 mg·kg~(-1)·d~(-1)) and E group for pathological control treated with high dosage resveratrol (16 mg·kg~(-1)·d~(-1)). At the end of the 12th week, aorta were analyzed by histopathological examination, and apoptotic cell were detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling. Results The rabbit atherosclerosis model had been successfully established with high fat diet. There were severe pathological lesions in group B. Apoptotic index was significantly increased in group B than in group A C 10. 62 ± 1. 53 vs. 2. 21 ± 0.31, P < 0. 01). Resveratrol could inhibit apoptosis of cells in atherosclerotic plaque in a dose dependent manner, and the apoptotic indexes were significantly decreased in group C, D and E compared with group B (9. 07 ± 1.41, 7. 55 ± 0. 96 and 5. 41 ± 1. 00 vs. 10. 62 ± 1. 53, respectively, all P < 0. 01). Conclusions Resveratrol can inhibit apoptosis in atherosclerotic plaque of rabbits, which may be involved in its anti-atherosclerotic effects.