谷胱甘肽硫基转移酶μ-3在前列腺癌中的表达及意义
Expression and significance of glutathione S-transferase mu 3 in prostate cancer
目的:探讨谷胱甘肽硫基转移酶μ-3( glutathione S-transferase mu 3,GSTM3)在前列腺癌中的表达及意义。方法2012年9月至2013年9月应用二维荧光差异凝胶电泳技术筛选前列腺癌标本中的差异蛋白,并利用质谱分析验证前期表达谱基因芯片结果获得的GSTM3。选取28例行根治性前列腺切除术患者的病理标本。患者年龄<60岁22例,≥60岁6例;PSA 水平<4μg/L 5例,≥4μg/L 23例;Gleason评分<7分26例,≥7分2例;TNM分期<T2a期18例,≥T2a期10例。采用实时荧光定量PCR法检测前列腺癌组织及其癌旁组织中GSTM3的表达情况,分析GSTM3与患者临床病理参数的相关性。结果二维荧光差异凝胶电泳获得前列腺癌与癌旁组织的双向凝胶电泳图谱,结果显示GSTM3为差异表达的蛋白,GSTM3蛋白在前列腺癌组织中表达下调,与癌旁组织比较差异有统计学意义( P<0.05)。实时荧光定量PCR法检测结果显示,GSTM3在癌旁组织中的表达(8.12±0.51)显著高于癌组织(7.18±0.54),差异有统计学意义(P<0.05)。 GSTM3的表达与患者的年龄、PSA水平、Gleason评分、TNM分期均无显著相关性(P>0.05),在不同PSA水平分组中GSTM3表达差异无统计学意义(P>0.05)。结论与癌旁组织比较,GSTM3在前列腺癌组织中表达下调,但GSTM3的表达与前列腺癌的恶性程度无关。
更多Objective To explore the role and clinical significance of GSTM 3 ( glutathione S-trans-ferase mu 3) expression in prostate cancer (PCa). Methods We had used the two-dimensional fluores-cence difference gel electrophoresis ( 2D-DIGE) and mass spectral analysis to further verify the microarray data of mRNA expression profiling discovered .GSTM3 mRNA level was detected by Rael-time Quantitative PCR ( RT-QPCR) in 28 pairs of prostate cancer tissue and benign tissue .The relationship of GSTM 3 level with the serum PSA level and the clinical feature of PCa were analyzed . Results In 2D-DIGE study, we found that the expression of GSTM 3 protein in adjacent tissues was significantly higher than that in PCa tis-sues (P<0.05).RT-QPCR results showed that GSTM3 in adjacent tissues (8.12±0.51) was significantly higher than that in PCa tissues (7.18±0.54) (P<0.05).There was no significant difference of GSTM3 ex-pression in different serum PSA packets ( P>0.05) and prostate cancer clinical pathological parameters ( P>0.05). Conclusions GSTM3 expression is down-regulated in PCa tissues, and we may identify PCa by detecting the GSTM 3 expression .
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