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双歧杆菌黏附素对大鼠肠缺血再灌注损伤的防护作用

The protective effects of bifidobacterial adhesin on ischemic reperfusion injury of intestine in rats

摘要:

目的 研究双歧杆菌分泌型黏附素对大鼠缺血再灌注(I/R)后肠黏膜屏障的防护作用。方法 雄性SD大鼠72只随机分为假手术组(24只)、I/R模型组(24只)和黏附素预处理组(预处理组,24只)。建模成功后6h及1、4、7d,各组分别取6只大鼠剖杀,观察小肠组织病理改变,并检测各时间点血中TNFα、IL-6、IL-10、二胺氧化酶(DAO)和D-乳酸的活性和含量。结果 I/R模型组血中TNFα、IL-6、DAO和D-乳酸水平在各时间点均高于假手术组(P值均<0.05),IL-10无明显差异;预处理组各时间点IL-6和DAO水平均明显低于I/R模型组(P值均<0.05),术后1d的TNFα浓度及术后4d和7d的血浆D-乳酸浓度也低于I/R模型组(P值均<0.05);预处理组的小肠病理改变较I/R模型组减轻(Chiu氏评分:6 h,3.22±0.22比3.57±0.20;1d,3.77±0.13比3.90±0.12;4 d,2.93±0.23比3.07 ±0.21;7 d,2.10±0.30比2.22±0.17,P值均<0.05)。结论 双歧杆菌黏附素对I/R后大鼠肠黏膜屏障具有防护作用,能减轻肠缺血再灌注损伤。

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Objective To investigate the protection effect of bifidobacterial adhesin for intestine ischemia/reperfusion (I/R) injury on gut barrier function in rat.Methods Seventy-two male SD rats were randomly divided into sham operation group (n =24), I/R model group (n =24) and pretreatment group of bifidobacterial adhesin (pretreatment group, n = 24).Six rats were anatomized at 6 h, 1 d, 4 d and 7d after inducing I/R model in each group, respectively.The pathological changes of the terminal ilea and the blood levels of TNFα, IL-6, IL-10, diamine oxidase (DAO), and the activity and content of D-lactic acid were observed.Results The blood levels of TNFα, IL-6, DAO and D-lactic acid in I/R model group were significantly higher than sham operation group at all time points (P <0.05) , while the blood level of IL-10 was no significantly change.The activity of IL-6 and DAO in pretreatment group was significantly lower than I/R model group at all time points (P < 0.05), the blood level of TNFαt in pretreatment group was significantly lower than I/R model group at 1 d, the blood level of D-lactic was significantly lower than I/R model group at 4 d and 7 d (P < 0.05). Intestinal pathological damages were obviously milder in pretreatment group than I/R model group at all time points (Chiu's pathological scores: 6 h, 3.22 ±0.22 vs 3.57 ±0.20;1 d,3.77 ±0.13 vs 3.90 ±0.12;4 d,2.93 ±0.23 vs 3.07 ±0.21;7 d,2.10 ±0.30 vs 2.22 ±0.17,all P < 0.05).Conclusion The pretreatment of bifidobacterial adhesin could protect the intestinal mucosa from I/R injury, and alleviate intestinal ischemic reperfusion injury.

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