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目的 探讨银屑病患者甘露糖结合凝集素(MBL)基因多态性及血清水平与疾病的关系.方法 寻常性银屑病患者67例,健康对照组69例.用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)法检测MBL基因第54号密码子基因多态性,ELISA法测定血清MBL浓度.结果 67例银屑病患者GGC/GAC基因型频率高于健康对照组,GGC/GGC基因型频率低于健康对照组,两组比较,差异有统计学意义(x2=10.36,P＜0.05)；银屑病组GAC等位基因频率增高,GGC等位基因频率降低,两组差异有统计学意义(x2=8.31,P＜0.05).GAC型等位基因与银屑病显著相关(OR=3.383,95％置信区间1.585～7.211,P＜0.05).银屑病组与健康对照组血清MBL浓度分别为(2.1937±0.8163) mg/L和(3.2695±1.2058) mg/L,银屑病组显著低于健康对照组(t=6.11,P＜0.05).结论 MBL与银屑病的发病可能存在一定关系.

Objective To determine the gene polymorphism and serum concentration of mannose-binding lectin (MBL) in patients with psoriasis,and to analyze the relationship between MBL and psoriasis.Methods Totally,67 patients with psoriasis vulgaris and 69 healthy human controls were enrolled in this study.Venous blood samples were obtained from all the subjects.Genomic DNA was extracted,and PCR-restriction fragment length polymorphism (PCR-RELP) analysis was conducted to determine the polymorphism at codon 54 of the MBL gene.Enzyme-linked immunosorbent assay was performed to measure the serum level of MBL.A chi-square goodness-of-fit test was carried out to evaluate Hardy-Weinberg equilibrium,t test to compare the serum concentration of MBL,and chi-square test to compare the frequency of genotypes and alleles of MBL gene codon 54.Results The patients with psoriasis showed higher frequency of GGC/GAC heterozygote but lower frequency of GGC/GGC homozygote (x2 =10.36,P ＜ 0.05),together with increased frequency of GAC allele but decreased frequency of GGC allele (x2 =8.31,P ＜ 0.05),at codon 54 of the MBL gene compared with the healthy controls.The variant allele GAC at codon 54 of the MBL gene was markedly associated with psoriasis (OR =3.383,95％ CI 1.585-7.211,P ＜ 0.05).The serum concentration of MBL was (2.193 7 ± 0.816 3) mg/L in patients with psoriasis,significantly lower than that in the healthy controls ((3.269 5±1.205 8) mg/L,t=6.11,P＜ 0.05).Conclusion MBL might be associated with the pathogenesis of psoriasis to some degree.