一例噬血细胞综合征与人疱疹病毒7型感染相关性的报告
One case of correlation between the human herpesvirus 7 DNA load and the hemo hagocytic syndrome
目的 研究人疱疹病毒7型(HHV-7)病毒感染与噬血细胞综合征发生的关系,为临床噬血细胞综合征患者诊断和抗病毒的治疗提供依据.方法 收集噬血细胞综合征患者不同治疗期外周血,提取DNA,合成HHV-7引物,PCR扩增片段,基因序列分析,荧光定量PCR法测定病毒载量,化学发光法测定血液铁蛋白的浓度,分析二者的相关性.结果 经PCR扩增和基因序列分析在噬血细胞综合征外周血中检测到HHV-7基因片段,其HHV-7病毒载量随着血液铁蛋白的浓度而变化.结论 HHV-7感染可以继发引起噬血细胞综合征并且与HHV-7病毒载量有关.
更多Objective To research the relationship between human herpesvirus 7 (HHV-7) viral Load and the etiopathogenisis of hemophagocytic syndrome, in order to provide evidence for the clinical diagnosis of hemophagocytic syndrome and anti-virus therapy. Methods Peripheral blood of patient with hemophagocytic syndrome during different treatment periods, extracted DNA, Syntheticed the primers of HHV-7,gene sequence of PCR amplified fragments detected, determined HHV-7 viral Load by Real - time fluorescent quantitative PCR and the ferritin concentration in peripheral blood detected by chemiluminescence. Result The sequence result indicated that PCR amplified fragment was a part of HHV-7 gene, the ferritin concentration viried with the load of HHV-7. Conclusion The occurrence of hemophagocytic syndrome is connetted with the load of HHV-7.
More- 浏览:324
- 被引:0
- 下载:80
相似文献
- 中文期刊
- 外文期刊
- 学位论文
- 会议论文