检索历史 清除

目的 观察中胚叶特异性转录子(MEST)在鼠成骨细胞中的表达对细胞迁移及增殖能力的影响.方法 取鼠成骨细胞进行培养,利用siRNA干扰片段下调MEST在成骨细胞中的表达,采用Transwell实验检测细胞的迁移能力,细胞计数试剂盒(CCK-8)法及常用增殖指标细胞核增殖抗原(Ki-67)检测细胞的增殖能力.结果 MEST表达下调后,成骨细胞的迁移数目为(10.0±1.9)个,明显低于空载组[(23.5±2.1)个]及空白对照组[(25.2±1.8)个],差异有统计学意义(P＜0.05);同时,CCK-8实验表明成骨细胞的增殖能力明显减弱,Ki-67表达明显降低.结论 MEST表达对成骨细胞具有促迁移和增殖的作用.

Objective To investigate the effect of mesoderm specific transcript (MEST) expression on the migration and proliferation of rat osteoblasts.Methods siRNA interference fragments were used to knock down the MEST expression in osteoblasts.Transwell assay was used to detect the migration ability,and cell counting kit-8 (CCK-8) and the detection of proliferation cell nuclear antigen (Ki-67) proliferation index were used to assess the proliferation ability.Results After MEST down-regulation,the number of migratory osteoblasts was (10.0 ± 1.9),significantly less than the mock-vehicle group (23.5 ±2.1) and control group (25.2 ± 1.8),with a statistically significant difference (P ＜ 0.05).Meanwhile,CCK-8 assay revealed that MEST down-regulation significantly reduced the proliferation ability of osteoblasts,and Ki-67 expression was also significantly decreased.Conclusion MEST expression can promote the migration and proliferation of osteoblasts.