骨桥蛋白对大肠癌细胞间隙连接通信的影响
Effects of osteopontin on gap junctional intercellular communication in colon cancer cell lines
目的 检测骨桥蛋白( Osteopontin,OPN)转染后大肠癌SW-480细胞株的细胞间隙连接通信( gap junctional intercellular communication,GJIC)改变。方法 以OPN真核表达质粒转染大肠癌SW-480细胞株后,利用激光共聚焦显微镜(laser scanning confocal microscope,LSCM),运用荧光淬灭后恢复技术(flurescence redistribution after photobleaching,FRAP)检测OPN转染后SW-480细胞的GJIC功能变化。结果 转染OPN后,高表达OPN的SW-480-pcDNA3.1(+)-OPN细胞株淬灭5 min 后荧光恢复率为24.65%±4.08%,而对照组SW-480-pcDNA3.1(+)为44.74% ±6.23%,P<0.001;10 min后荧光恢复率SW-480-pcDNA3.1(+)-OPN仅为25.98%±4.48%,SW-480-pcDNA3.1(+)已上升至64.92%±5.39%,P<0.001。转染后高表达OPN的SW-480细胞,GJIC功能被抑制,细胞之间通信减弱,淬灭后恢复率相比较对照组明显降低。结论 OPN减弱大肠癌细胞间的通信能力,可能与大肠癌细胞的侵袭转移相关。
更多ObjectiveLiver metastasis is a major cause of mortality in patients with colorectal cancer. However, the mechanisms underlying this process remain largely unknown. Osteopontin (OPN) is a secreted phosphorylated glycoprotein known to be involved in tumor migration and metastasis. Gap junction intercellular communication (GJIC) is a pathway of direct cell to cell communication. This study analyzed gap junctional intercellular communication(GJIC)changes in colon cancer SW-480 cell lines after osteopontin had been transfected in. MethodsTransfect sense-osteopontin eukaryotic expression plasmids into SW-480 cell lines and detect the GJIC changes in two different cell lines by flurescence redistribution after photobleaching ( FRAP).ResultsCompared with control, signal exchange is impaired and GJIC function is inhibited in SW-480-pcDNA3. 1 ( + )-OPN cell lines. FRAP after 5 min in SW-48O-pcDNA3. 1 ( + )-OPN is 24. 65% ± 4. 08%, the control SW-480-pcDNA3. 1 ( + ) is 44. 74% ± 6. 23%, P < 0. 001 ;FRAP after 10 min in SW-480-pcDNA3. 1 ( + )-OPN is 25.98% ±4.48%, while SW-480-pcDNA3. 1 is up to 64. 92% ± 5.39%, P < 0.001. ConclusionOPN could inhibit GJIC function of colon cells to accelerate the metastatic process in colorectal cancer.
More- 浏览:217
- 被引:2
- 下载:8
相似文献
- 中文期刊
- 外文期刊
- 学位论文
- 会议论文