一站式CT能谱灌注成像参数与兔VX2乳腺癌腋窝淋巴结微淋巴管密度及血管内皮生长因子C表达的关系
Relationship between one-stop CT spectral perfusion imaging parameters and expression of lymphatic microvessel density and vascular endothelial growth factor-C in axillary lymph nodes of rabbit VX2 breast cancer
目的 分析一站式CT能谱灌注成像表现及其与兔VX2乳腺癌腋窝转移性淋巴结微淋巴管密度(MLVD)、血管内皮生长因子C(VEGF-C)表达的相关性.方法 30只新西兰纯种雌性大白兔建立兔VX2乳腺癌腋窝淋巴结转移模型,行一站式CT能谱灌注扫描,对应影像标记取腋窝淋巴结,苏木精-伊红(HE)染色法观察形态及免疫组化EnVision法定量分析VEGF-C表达并计MLVD.采用Pearson相关分析转移性淋巴结能谱灌注参数特征及MLVD、VEGF-C表达的相关性.结果 24只实验兔建模成功于第28天行一站式CT扫描.转移性淋巴结共39枚,转移性淋巴结VEGF-C为20.0%±2.8%,MLVD为(12.5±3.5)个.淋巴结血流量(BF)、动脉期曲线斜率(APλHU)、静脉期碘浓度(ICVP)、静脉期标准化碘浓度(NICVP)、静脉期曲线斜率(VPλHU)与VEGF-C、MLVD均呈正相关性(P<0.05),动脉期碘浓度(ICAP)与MLVD值呈正相关性(P=0.027),与VEGF-C表达无相关性(P=0.386).淋巴结血容量(BV)、动脉期标准化碘浓度(NICAP)与VEGF-C、MLVD表达均无相关性(均P>0.05).淋巴结VPλHU与MLVD的相关性最高(r=0.448,P=0.001).结论 一站式CT能谱灌注成像定量参数与病理指标具有良好相关性,可反映转移淋巴结内的淋巴管生成情况.
更多Objective To analyze the correlation between quantitative parameters of one-stop spectral perfusion computed tomography imaging and the expression level of vascular endothelial growth factor-C(VEGF-C) and MLVD in metastatic lymph nodes of rabbit VX2 breast cancer.Methods Thirty New Zealand purebred female rabbits were used to establish the lymph node metastasis model of rabbit VX2 breast cancer,one-stop spectral and perfusion CT imaging protocol was performed.The axillary lymph nodes were selected for corresponding image markers.The observed morphology of conventional HE staining and the EnVision method were used to quantitatively analyze VEGF-C expression and calculate MLVD.Pearson linear correlation was used to analyze the perfusion parameters of metastatic lymph node energy spectrum and the correlation of MLVD and VEGF-C expression.Results Twenty-four experimental rabbits were successfully modeled and performed a one-stop CT scan on the 28th day.A total of 39 metastatic lymph nodes were included.The VEGF-C of metastatic lymph node was 20.0%±2.8%,and the MLVD was 12.5±3.5.There was a positive correlation between BF,APλHU,ICVP,NICVP,VPλHU of metastatic lymph node and VEGF-C and MLVD (P<0.05).There was a positive correlation between lymph node ICAP and MLVD (P=0.027) and no correlation with VEGF-C expression (P=0.386).There was no correlation between BV,NICAP and VEGF-C,MLVD(P>0.05).The correlation between VPλHU and MLVD was higher (r=0.448,P=0.001).Conclusions One-stop CT spectral perfusion imaging quantitative parameters and pathological indicators have a good correlation,and it can reflect lymphatic vessel metastasis in lymph nodes.
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