LPS ligand and culture additives improve production of monomeric MD-1 and 2 in Pichia pastoris by decreasing aggregation and intermolecular disulfide bonding.
第一作者:
Kristen E,Mengwasser
第一单位:
Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom.
作者:
医学主题词
动物(Animals);抗原, 表面(Antigens, Surface);色谱法, 凝胶(Chromatography, Gel);克隆, 分子(Cloning, Molecular);培养基(Culture Media);二硫化物类(Disulfides);电泳, 聚丙烯酰氨凝胶(Electrophoresis, Polyacrylamide Gel);马(Horses);人类(Humans);配体(Ligands);脂多糖类(Lipopolysaccharides);淋巴细胞抗原96(Lymphocyte Antigen 96);表型(Phenotype);毕赤酵母(Pichia);蛋白质多聚化(Protein Multimerization);重组融合蛋白质类(Recombinant Fusion Proteins)
DOI
10.1016/j.pep.2010.11.018
PMID
21130168
发布时间
2021-10-20
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