Microfluidic screening and whole-genome sequencing identifies mutations associated with improved protein secretion by yeast.

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第一作者： Mingtao,Huang

第一单位： Department of Biology and Biological Engineering, Chalmers University of Technology, SE41296 Gothenburg, Sweden; Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology, SE41296 Gothenburg, Sweden;

作者： Mingtao,Huang ^[1] ; Yunpeng,Bai ^[2] ; Staffan L,Sjostrom ^[2] ; Björn M,Hallström ^[2] ; Zihe,Liu ^[1] ; Dina,Petranovic ^[1] ; Mathias,Uhlén ^[3] ; Haakan N,Joensson ^[2] ; Helene,Andersson-Svahn ^[2] ; Jens,Nielsen ^[4]

作者单位： Department of Biology and Biological Engineering, Chalmers University of Technology, SE41296 Gothenburg, Sweden; Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology, SE41296 Gothenburg, Sweden; ^[1] Novo Nordisk Foundation Center for Biosustainability, KTH Royal Institute of Technology, SE10691 Stockholm, Sweden; Science for Life Laboratory, KTH Royal Institute of Technology, SE17165 Solna, Sweden; Division of Proteomics and Nanobiotechnology, KTH Royal Institute of Technology, SE10691 Stockholm, Sweden; ^[2] Novo Nordisk Foundation Center for Biosustainability, KTH Royal Institute of Technology, SE10691 Stockholm, Sweden; Science for Life Laboratory, KTH Royal Institute of Technology, SE17165 Solna, Sweden; Division of Proteomics and Nanobiotechnology, KTH Royal Institute of Technology, SE10691 Stockholm, Sweden; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, DK2970 Hørsholm, Denmark. ^[3] Department of Biology and Biological Engineering, Chalmers University of Technology, SE41296 Gothenburg, Sweden; Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology, SE41296 Gothenburg, Sweden; Science for Life Laboratory, KTH Royal Institute of Technology, SE17165 Solna, Sweden; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, DK2970 Hørsholm, Denmark nielsenj@chalmers.se. ^[4]

关键词 droplet microfluidics protein secretion random mutagenesis systems biology yeast cell factories

主题词基因组, 真菌(Genome, Fungal);微流体学(Microfluidics);突变(Mutation);酿酒酵母蛋白质类(Saccharomyces cerevisiae Proteins)

DOI 10.1073/pnas.1506460112

PMID 26261321

发布时间 2022-01-29

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