摘要目的:探讨虎杖苷对脂多糖（LPS）诱导的皮肤成纤维细胞凋亡、氧化应激及炎性反应的保护作用。方法:2021年1月至4月，以5 mg/L LPS刺激成纤维细胞建立实验模型，并随机分为4组：正常对照组细胞不接受任何处理；药物对照组细胞接受50 μmol/L虎杖苷处理；实验组细胞接受5 mg/L LPS处理；治疗组细胞接受5 mg/L LPS及50 μmol/L虎杖苷处理。细胞增殖检测试剂盒（CCK-8）检测细胞活力；末端脱氧核苷酸转移酶介导的dUTP缺口末端标记测定法［terminal dexynucleotidyl transferase（TdT）-mediated dUTP nick end labeling，TUNEL］检测细胞凋亡；试剂盒检测细胞丙二醛（malonaldehyde，MDA）及超氧化物歧化酶（superoxide dismutase，SOD）含量；酶联免疫吸附剂测定（ELISA）检测培养基肿瘤坏死因子（TNF）-α及白介素（IL）-6含量。结果:与正常对照组比较，实验组细胞凋亡率、MDA含量、培养基TNF-α及IL-6含量分别显著增加为（12.70±0.11）%、（3.38±0.29）nmol/（mg·pr）、（483.5±38.4）ng/L及（784.4±49.6）ng/L，细胞活力及SOD含量分别显著下降为（78.00±5.90）%及（11.5±1.3）U/（mg·pr）。与实验组比较，治疗组细胞凋亡率、MDA含量、培养基TNF-α及IL-6含量分别显著下降为（7.50±0.08）%、（2.18±0.19）nmol/（mg·pr）、（283.6±25.3）ng/L及（518.5±42.2）ng/L，细胞活力及SOD含量分别显著增加为（89.00±7.50）%及（19.1±2.2）U/（mg·pr）。结论:虎杖苷显著抑制LPS诱导的成纤维细胞凋亡、氧化应激及炎性反应，并促进细胞增殖。

abstractsObjective:To investigate the protective effect of polydatin on the apoptosis, oxidative stress, and inflammatory response of skin fibroblasts induced by lipopolysaccharide (LPS).Methods:From January to April, 2021, the fibroblasts were stimulated by 5 mg/L LPS to establish the experimental models, and were randomly divided into 4 groups: the cells in the normal control group received no treatment; the cells in the drug control group were treated with 50 μmol/L polydatin; the cells in the experimental group were treated with 5 mg/L LPS; and the cells in the treatment group were treated with 5mg/L LPS and 50 μmol/L polygonin. The cell proliferation and cytotoxicity assay kit (CCK-8) was used to detect the cell viability. The cell apoptosis was detected by terminal dexynucleotidyl transferase(TdT)-mediated dUTP nick end labeling (TUNEL) assay. The malonaldehyde (MDA) and superoxide dismutase (SOD) contents in the cells were detected by the commercial kits. The enzyme-linked immunosorbent assay (ELISA) kits were used to detect the levels of tumor necrosis factor-α (TNF-α)and interleukin-6 (IL-6) in the culture medium.Results:Compared with those in the normal control group, the apoptosis rate and the contents of MDA, TNF-α, and IL-6 in the culture medium were respectively increased to (12.7±0.11)%, (3.38±0.29) nmol/(mg·pr), (483.5±38.4) ng/L, and (784.4±49.6) ng/L and the cell viability and SOD content were respectively decreased to (78.00±5.90)% and (11.5±1.3) U/(mg·pr) in the experimental group. Compared with those in the experimental group, the cell apoptosis rate and the contents of MDA, TNF-α, and IL-6 in the culture medium were respectively decreased to (7.5±0.08)%, (2.18±0.19) nmol/(mg·pr), (283.6±25.3) ng/L, and (518.5±42.2) ng/L and the cell viability and SOD content were respectively increased by (89.00±7.5)% and (19.1±2.2) U/(mg·pr) in the treatment group.Conclusion:Polygonin significantly inhibits the apoptosis, oxidative stress, and inflammatory response of fibroblasts induced by LPS, and promotes cell proliferation.