摘要目的:检测胃癌组织中同源结构域相互作用蛋白激酶-2（homeodomain-interacting protein kinase-2，HIPK2）及多药耐药基因1（multiple drug resistance-1，MDR-1）的表达，并探讨其临床意义。方法:收集2011年8月1日至2015年10月1日滨州医学院附属医院手术切除的胃癌组织156例，男99例，女57例，年龄36～79岁，中位年龄56岁。免疫组织化学法检测HIPK2、MDR-1表达，分析HIPK2表达与临床病理参数的关系及与MDR-1表达的相关性。组间比较采用 χ2检验；基因间相关性分析采用Pearson相关性分析。 结果:免疫组织化学结果显示，胃癌组织中HIPK2及MDR-1阳性表达率分别为29.5%（46/156）、46.8%（73/156）。不同Lauren分型、肿瘤部位、淋巴管侵犯、淋巴结转移、性别患者HIPK2阳性表达率比较，差异均无统计学意义（均 P>0.05）；肿瘤长径≥6.5 cm、MDR-1表达阳性的患者HIPK2阳性表达率低于肿瘤长径<6.5 cm、MDR-1表达阴性的患者（均 P<0.05）；HIPK2表达与MDR-1的表达呈负相关性（ r=-0.167， P<0.05）。 结论:胃癌组织中HIPK2呈低表达，与MDR-1的表达呈负相关，可能参与胃癌的发生、发展过程。

abstractsObjective:To detect the expressions of homodomain-interacting protein kinase-2 (HIPK2) and multiple drug resistance-1 (MDR-1) in gastric cancer tissue, and to explore their clinical significance.Methods:One hundred and fifty-six cases of gastric cancer tissue surgically resected in Binzhou Medical University Hospital from August 1, 2011 to October 1, 2015 were collected, including 99 males and 57 females who were 36-79 years old, with a median age of 56. The immunohistochemical method was used to detect the expressions of HIPK2 and MDR-1. The relationship between HIPK2 expression and clinicopathological parameters and the correlation with MDR-1 expression were analyzed. χ2 test was used for the comparison between the groups; Pearson correlation analysis was used for the correlation analysis between the genes. Results:The immunohistochemistry results showed that the positive expression rates of HIPK2 and MDR-1 in the gastric cancer tissue were 29.5% (46/156) and 46.8% (73/156), respectively. There were no statistical differences in the HIPK2 positive-expression rate between the patients with different Lauren types, tumor sites, lymph-vessel invasion, lymph node metastasis, and genders (all P>0.05). The HIPK2 positive-expression rates in the patients with maximum tumor diameter≥6.5 cm and positive MDR-1 expression were lower than those in the patients with maximum tumor diameter<6.5 cm and negative MDR-1 expression (both P<0.05). HIPK2 expression was negatively correlated with MDR-1 expression ( r=-0.167; P<0.05). Conclusion:HIPK2 is lowly expressed in gastric cancer tissue and negatively correlated with the expression of MDR-1, and may be involved in the processes of gastric cancer development and progression.