实时荧光核酸恒温扩增法检测泌尿生殖道淋球菌感染
Clinical analysis of simultaneous amplification and testing technology in the detection of Neisseria gonorrhoeae from genitourinary specimens
摘要目的 评价实时荧光核酸恒温扩增检测技术检测淋球菌尿液和拭子样本的特性.方法 运用淋球菌培养和实时荧光核酸恒温扩增试验平行检测205份性病门诊患者生殖道拭子样本,同时对对应的205份尿液样本进行实时荧光核酸恒温扩增检测,用实时荧光PCR法对两种试验比较产生的差异样本进行检测.结果 实时荧光核酸恒温扩增法平行检测同一来源的拭子样本和尿液样本,检测结果完全一致.与金标准淋球菌培养法相比,实时荧光核酸恒温扩增试剂盒对拭子和尿液样本的检测灵敏度均为100%,特异性均为94.5%.实时荧光PCR结果显示,7份差异样本中,6份拭子样本结果与实时荧光核酸恒温扩增试剂盒结果一致.结论 实时荧光核酸恒温扩增试剂盒在检测淋球菌临床拭子及尿液样本时具有较高的灵敏度和特异性,而且耗时短,为淋球菌的实验室诊断提供新的检测方法.
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abstractsObjective To evaluate the performance of simultaneous amplification and testing (SAT) kit for detecting Neisseria gonorrhoeae(NG) from swab and urine specimens. Methods A total of 205 clinical samples of male urethral swab, female endocervical swab and FCU (first-catch urine) were collected from symptomatic and asymptomatic STD clinic clients. The swab samples were tested by both SAT and culture,while FCU samples by SAT only. The samples with inconsistent test results were further re-tested by another PCR. Results NG-SAT yielded the sensitivity and specificity of 100% and 94.5%, respectively, for both swab and urine samples. Six of 7 specimens with divergent results between SAT and culture showed consistent results between SAT and PCR. Conclusion These results indicate that SAT is sensitive, specific, and timesparing in the detection of NG, which may provide a novel approach to the laboratory diagnosis of gonorrhea.
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