摘要玻璃化冷冻保存技术通过超快速冷却将生物样本中的液态水转化为玻璃态固体。主要聚焦玻璃化冷冻保存技术的核心研究如冷冻保护剂配方优化、温控方法改进、载体创新设计和复温技术改进等，总结其在器官保存、肿瘤研究、生殖医学和稀有细胞保存等领域的应用现状与问题，结合技术原理与玻璃态检测方法，为优化技术体系和拓展应用场景提供理论依据，推动其在临床及生物样本库的规模化应用。

abstractsVitrification cryopreservation technology converts the liquid water in biological samples into glassy solids through ultra-rapid cooling. In this review, the core research of vitrification cryopreservation technology was mainly reviewed, such as the optimization of cryoprotectant formulations, the improvements to temperature control methods, the innovative carrier designs and the improvements to rewarming technology. The application status and issues in the fields of organ preservation, tumor research, reproductive medicine and rare cell preservation were also summarized. By combining the technical principles with glassy state detection methods, a theoretical basis was provided for optimizing the technical system and expanding application scenarios, thereby promoting its large-scale application in clinical and biological sample libraries.