摘要自噬是当前生物医学领域的研究热点之一,因为自噬过程与多种疾病的发病机制相关.自噬流是由隔离膜的形成至自噬溶酶体完成水解功能的过程,可反映多步骤且动态发生的完整自噬过程.因此,自噬流可以作为自噬水平的度量指标,并且通过动态和静态相结合的实验检测手段,实现对自噬流的定性、定量检测,从而更加准确、客观地反映自噬水平.笔者拟就自噬检测的研究进展进行综述,并且着重对各种自噬流的检测方法进行介绍,以期为广大相关研究者提供技术支持.

abstractsAutophagy is one of hotspots in the biomedical field.It has been shown that autophagy is related to the pathogenesis of various diseases.The autophagic flux is a process from the formation of the barrier membrane to the autolysis of the lysosome to complete the hydrolysis function,which is a complete,multistep and dynamic autophagy process.Therefore,autophagic flux can be used as a measure of autophagy level,and qualitative and quantitative detection of autophagic flux can be achieved by combining experimental methods of dynamic and static.It can reflect the level of autophagy more accurately and objectively.This article intends to give a brief introduction to the detection of autophagy,and especially describes various detection methods of autophagic flux,in order to provide technical support for the majority of related researchers.