摘要目的:探讨采用复苏等量分装的标准贮备菌株培养18～24 h后配制的菌悬液作为生物制品微生物验证用菌液的可行性。方法:制备等量分装的标准贮备菌株，选择保藏0、3、6、9、12、13个月对其复苏后菌液进行菌落计数，对计数结果进行单因素方差分析。结果:分析显示，储存3、6、9、12、13个月的金黄色葡萄球菌、大肠埃希菌、铜绿假单胞菌、白色念珠菌计数与0个月比较无显著差异， F值分别为2.24、2.21、0.58、1.59， P值均大于0.05。计数结果稳定。 结论:采用等量分装的标准贮备菌株复苏后配制菌悬液，可作为生物制品微生物验证用菌液，菌液浓度稳定。

abstractsObjective:To evaluate the feasibility of preparing bacterium suspension by recovering the standard bacterium in equal volume and culturing 18-24 h for microbial verification.Methods:Standard bacteria were prepared in equal volume. Then the recovered strains were counted after storing for 0, 3, 6, 9, 12, 13 months. The colony counting results were analyzed by single-factor analysis of variance.Results:Compared with 0 month, the counts of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans after storing for 3, 6, 9, 12, 13 months were stable and of no statistically significant difference, with F values of 2.24, 2.21, 0.58 and 1.59, respectively, and all P values>0.05. Conclusion:Using recovered standard bacterium in equal volume to prepare the bacterium suspension for microbial verification of biological products is feasible, and bacterium concentration is stable.