摘要目的:建立白背叶中1个黄酮成分的提取分离方法及其薄层色谱鉴别方法。方法:用95%乙醇回流提取白背叶，旋蒸回收乙醇，用硅胶拌匀，以石油醚洗脱、乙酸乙酯回流提取，回收浓缩乙酸乙酯部位。取乙酸乙酯部位，上聚酰胺柱，用水和甲醇梯度洗脱，收集60%乙醇馏分，进一步分离纯化；以大波斯菊苷为对照品，白背叶为对照药材，硅胶G板为吸附剂，以三氯甲烷-甲醇-甲酸(10∶3∶0.3)为展开剂，进行薄层色谱鉴别。结果:从白背叶中分离得到大波斯菊苷，建立了白背叶薄层鉴别方法，斑点清晰，分离效果好。结论:该方法可准确、快速对白背叶进行鉴别，可更好地控制白背叶药材质量。

abstractsObjective:To establish the extraction and separation method of flavone from the Folium malloti apeltae and TLC identification method. Methods:The Folium malloti apeltae was extracted by 95% ethyl alcohol, and the ethanol recovery by rotary distillation. The extracted Folium malloti apeltae was mixed well with silicone. It was eluted with petroleum ether and extracted with ethyl acetate. Then, the concentrated ethyl acetate fraction was recovered. The ethyl acetate fraction was separated and purified on a polyamide column with gradient elution of water and methanol. The TLC identification was carried out with daposthride as the reference substance, the leaves as the reference material, the silica gel G plate as the adsorbent, and chloroform methanol formic acid (10:3:0.3) as the developing agent. Results:Apigenin-7-O-glucoside obtained from Folium malloti apeltae. TLC identification method of Folium malloti apeltae was established with detectable spots and satisfactory separating result. Conclusions:This method to identify Folium malloti apeltae is accurate and rapid, which can be used for the quality control of Folium malloti apeltae.