摘要Objective:To investigate the antioxidant property of purified bioactive compound from sponge associated bacteria Bacillus species. <br> Methods:The potential active compound was subjected for assay of total antioxidant activity,α,α-diphenyl-α-picrylhydrazyl (DPPH) radical scavenging activity, nitric oxide radical scavenging activity, hydrogen peroxide (H2O2) scavenging activity and total reducing power. Further, the 16S rRNA gene sequence was carried out to identify the sponge symbiotic bacteria. Results:The results showed linear increase of total antioxidant activity, DPPH radical scavenging activity, nitric oxide radical scavenging activity, H2O2 scavenging activity and total reducing power. IC50 value of the active compound for DPPH activity, H2O2 scavenging activity, nitric oxide scavenging activity was recorded as 15.025μg/mL, 23.73μg/mL, and 41.70μg/mL respectively. The potential strain was identified as Bacillus species from GenBank database (GenBank Accession number JX985748). <br> Conclusions: The present study has reported the antioxidant property of purified bioactive compound from sponge associated Bacillus species. The report will be helpful to pharmaceutical and antioxidant researchers for further studies.