换一批
换一批Downregulation of alpha-fetoprotein siRNA inhibits proliferation of SMMC-7721 cells
摘要：
AIM: To study the function of α-fetoprotein (AFP) in SMMC-7721 hepatoma cells.METHODS: A hairpin siRNA expressing plasmid pSilencer3.0-H1-afp was constructed and transfected into SMMC-7721 cells with Lipofectamine 2000. The expression of AFP was monitored by real-time RT-PCR and immunoassays, its effect on SMMC-7721 cell proliferation and cell death was detected by MTT and fluorescenceactivated cell sorter (FACS).RESULTS: The AFP-siRNA expressing plasmid downregulated the expression of AFP obviously (about 34%), and inhibited SMMC-7721 cell proliferation, but did not induce apoptosis.CONCLUSION: Downregulation of AFP siRNA inhibits proliferation of SMMC-7721 cells, but cannot cause apoptosis.
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作者单位：
Central Laboratory, Jinan Central Hospital, Clinical Medical College of Shandong University, Jinan 250013, Shandong Province, China
[1]
Laboratory of Clinical Molecular Biology, The Second Hospital of Shandong University, Jinan 250015, Shandong Province, China
[2]
Shandong Provincial Hospital, Jinan 250020,Shandong Province, China
[3]
期刊：
《世界胃肠病学杂志（英文版）》2005年11卷38期 6053-6055页
SCIMEDLINEISTIC
分类号：
R3
发布时间：
2006-07-31
基金项目：
the Natural Science Foundation of Shandong Province
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