Downregulation of alpha-fetoprotein siRNA inhibits proliferation of SMMC-7721 cells

二维码有效期 120s

摘要AIM: To study the function of α-fetoprotein (AFP) in SMMC-7721 hepatoma cells.METHODS: A hairpin siRNA expressing plasmid pSilencer3.0-H1-afp was constructed and transfected into SMMC-7721 cells with Lipofectamine 2000. The expression of AFP was monitored by real-time RT-PCR and immunoassays, its effect on SMMC-7721 cell proliferation and cell death was detected by MTT and fluorescenceactivated cell sorter (FACS).RESULTS: The AFP-siRNA expressing plasmid downregulated the expression of AFP obviously (about 34%), and inhibited SMMC-7721 cell proliferation, but did not induce apoptosis.CONCLUSION: Downregulation of AFP siRNA inhibits proliferation of SMMC-7721 cells, but cannot cause apoptosis.

作者单位 Central Laboratory, Jinan Central Hospital, Clinical Medical College of Shandong University, Jinan 250013, Shandong Province, China ^[1] Laboratory of Clinical Molecular Biology, The Second Hospital of Shandong University, Jinan 250015, Shandong Province, China ^[2] Shandong Provincial Hospital, Jinan 250020,Shandong Province, China ^[3]

关键词 siRNAs AFP SMMC-7721 cell Proliferation Apoptosis

分类号 R3

发布时间 2006-07-31

基金项目

the Natural Science Foundation of Shandong Province（Z2003C01）

翻译满意度评价：

准确一般字义偏差语意偏离译文作用小提交