摘要目的 观察益骨胶囊含药血清在成骨-破骨细胞共育体系中对SD大鼠成骨细胞表达骨保护素(OPG)mRNA的影响.方法 取1d龄SD大鼠颅骨分离培养成骨细胞(OB)；取5d龄SD大鼠四肢长骨分离培养破骨细胞(OC).建立上清相通但细胞间不相互混杂的平面式成骨-破骨细胞共育体系,实验分为含药血清组和对照组.将10月龄SD雌性大鼠分为益骨胶囊灌胃组和生理盐水对照组,制备含药血清和对照血清.检测共育体系中成骨细胞OPG mRNA的表达.结果 含药血清组与对照组比较,OPGmRNA表达明显升高(8.2567±0.1118比3.3350±0.9854),差异有统计学意义(P＜0.01).结论 益骨胶囊含药血清在共育体系中通过刺激OB表达OPG来实现对OC的活性和凋亡的调节.

abstractsObjective To investigate the impact of Yigu capsule- containing serum on the expression of osteoprotegerin (OPG)mRNA in SD rat osteoblasts in an osteoblast and osteoclast co-culture system.Methods Osteoblasts (OBs) and osteoclasts (OCs) were isolated and cultured from the skulls of 1-day- old and the fetal long bones of 5- day- old SD rats for establishment of the serum interlinked but intercellular unmixed plane OB and OC co- culture system.The rats were then divided into medicinecontaining serum and control groups.Furthermore,the 10-month-old female SD rats were divided into Yigu capsule-gastric lavage and NS groups for preparations of medicine containing serum and control serum.FQPCR method was used to detect the OPG mRNA expression in OBs in the co- culture system.Results Compared with the control group,the medicine containing serum group had a significant increase in OPG mRNA expression (8.2567±0.1118 vs 3.3350±0.9854,P＜0.01).Conclusion In the co-culture system,Yigu capsule containing serum may regulate the activity and apoptosis of OC by stimulating OB to express OPG.