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目的 研究微RNA-214(miR-214)对舌癌Tca8113细胞迁移侵袭等生物学行为的影响及其起效机制.方法 建立Tca8113细胞miR-214模拟物(M)组-阴性对照模拟物(N)组及阴性对照(C)组.采用RT-PCR法检测各组miR-214水平,Western blot法检测膜型基质金属蛋白酶1(MT1-MMP)、血管内皮细胞生长因子(VEGF)的表达.采用CCK-8实验检测细胞生长抑制率.采用细胞划痕实验和Transwell实验检测抑制细胞侵袭转移的能力.结果 与对照组相比,miR-214模拟物干预Tca8113细胞后,M组miR-214表达水平明显升高(P＜0.05),MT1-MMP、VEGF蛋白表达水平降低,细胞迁移侵袭能力明显降低(P＜0.05).结论 miR-214可能通过降低MT1-MMP、VEGF蛋白表达,抑制舌癌Tca8113细胞的迁移侵袭能力.

Objective To explore the inhibitory effect and biological mechanism of the micro RNA-214 (miR-214) on the migration and invasion of human tongue cancer cell line Tca8113.Methods Group M (miR-214 mimics),Group N (negative control mimic) and Group C (negative control) of Tce8113 cells were established.The expression of miR-214 in each group was detected by real-time PCR.The expressions of MT1-MMP and VEGF in each group were detected by Western blot assay.CCK-8 assay was utilized to investigate the rate of cell growth inhibition.And cell migration and invasion were detected by cell scratch test and Transwell assay.Results Compared with the control groups,the miR-214 expression in Group M was significantly increased after Tca8113 cells were transfected by miR-214 mimics (P＜0.05).Also,the MT1 -MMP and VEGF protein levels in Group M was reduced,along with the significant reduction in cell migration and invasion ability (P＜0.05).Conclusion miR-214 may inhibit the migration and invasion of tongue cancer cells Tca8113 by down-regulating the expressions of MT1-MMP and VEGF proteins.