摘要目的 探讨高迁移率族蛋白A2(HMGA2)基因对卵巢颗粒细胞凋亡及PI3K/AKT信号通路的影响.方法 分离培养原代多囊卵巢综合征(PCOS)大鼠卵巢颗粒细胞,将HMGA2重组质粒转染细胞,同时转染空载体pcDNA3.1,并设置空白组,转染48h,通过免疫印迹检测转染后的细胞HMGA2蛋白表达.MTT法、流式细胞术检测细胞活力和凋亡率.免疫印迹检测凋亡相关的Bcl-2、Bax和Cleaved caspase3及PI3K/AKT信号通路PI3K、AKT和p-AKT蛋白表达.结果 转染HMGA2重组质粒的卵巢颗粒细胞HMGA2表达明显升高(P＜0.05).过表达HMGA2可明显抑制卵巢颗粒细胞活力,诱导细胞凋亡,下调Bcl-2、PI3K和p-AKT表达,上调Bax和Cleaved caspase3表达(P＜0.05).结论 HMGA2基因可诱导卵巢颗粒细胞凋亡,机制与抑制PI3K/AKT信号通路有关.

abstractsObjective To investigate the effect of high mobility group AT-hook 2 (HMGA2) gene on apoptosis of ovarian granulosa cells and PI3K/AKT signaling pathway.Methods The ovarian granulosa cells from primary polycystic ovary syndrome (PCOS) rats were isolated and cultured.HMGA2 recombinant plasmid was transfected into the cells and empty vector pcDNA3.1,respectively,and a blank group was established.After transfection for 48 h,Western blotting was used to examine HMGA2 protein expression in the transfected cells.Cell viability and apoptosis rate were measured by MTT assay and flow cytometry.Western blotting was used to determine the proteinexpression of apoptosis-related Bcl-2,Bax,Cleaved caspase 3 and PI3K/AKT signaling pathways PI3K,AKT and p-AKT.Results The expression level of HMGA2 in ovarian granulosa cells transfected with HMGA2 recombinant plasmid significantly increased (P＜0.05).Overexpression of HMGA2 could significantly inhibit the viability of ovarian granulosa cells,induce cell apoptosis,down-regulate the expression levels of Bcl-2,PI3K and p-AKT,and up-regulate the expression levels of Bax and Cleaved caspase 3 (P＜0.05).Conclusion HMGA2 gene may induce apoptosis of ovarian granulosa cells,and its mechanism is related to the inhibition of PI3K/AKT signaling pathway.