摘要目的 了解广州地区急性呼吸道感染(ARTIs)儿童中人偏肺病毒(HMPV)、人博卡病毒(HBoV)和腺病毒(ADV)的流行病学及临床特征.方法 采集2016年1月至2018年8月因ARTIs至广州市妇女儿童医疗中心就诊住院的患儿咽拭子等标本13597例,应用实时荧光探针RT?PCR方法检测HMPV、HBoV和ADV.结果 2016?2018年腺病毒的总检出率为7.81%(1 062/13 597);2016年腺病毒检出率为6.73%(290/4 310),2017年的检出率为6.24%(323/5178),2018年前8个月ADV的检出率为10.93%(449/4 109).2016?2018年偏肺病毒总检出率为2.29%(312/13 597),2016年HMPV检出率为0.91%,有5个月份没有检出病毒,2017年检出率为2.47%,2018年前8个月为3.53%.博卡病毒2016?2018年的总检出率为4.24%(576/13 597),2016年HBOV检出率为4.43%,2017年检出率为4.21%, 2018年前8个月份检出率为4.06%.2016?2018检测结果表明腺病毒感染主要以夏季感染为主,博卡病毒主要以秋季为主要的流行季节,偏肺病毒以春夏为流行高峰.三种病毒主要导致非重症社区获得性肺炎,博卡病毒和偏肺病毒感染引起的喘息性支气管肺炎比腺病毒的要高.腺病毒、偏肺病毒和博卡病毒感染≤2岁患儿感染率分别为63.84%、76.28%和86.98%.结论 腺病毒和博卡病毒是广州地区ARTIs儿童感染的重要病原体,偏肺病毒感染率相对较低.博卡病毒和偏肺病毒是诱发部分婴幼儿喘息性疾病的重要病原体.

abstractsObjective To investigate the epidemic and clinical features on human metapneumovims(hMPV)and human bocavirus(HBOV)among pediatric patients with acute respiratory infections(ARTIs)in Guangzhou. Methods A total of 13597 children with ARTIs from January 2016 to August 2018 were recruited. The pharyngeal swab specimens were collected. The ADV/hMPV and HBoV were detected by using real?time PCR. Results In 13597 children,1062 children(7.81%)were ADV positive on swab specimens,576 children (4.24%) were HBoV positive ,312 children (2.29%) were HMPV positive. The detection rates of ADV in 2016、2017 and 2018 year were 6.73%、6.24% and 10.93%, respectively. The detection rates of HBOV in 2016、2017 and 2018 were 4.43%、4.21% and 4.06%, respectively. The detection rates of HMPV in 2016、2017 and 2018 were 0.91%、2.47% and 3.53%, respectively,HMPV was not detected at five months of 2016 year. The epidemiological peaks of ADV and HBOV were mainly occurred in summer and autumn. The epidemiological peaks of HMPV were mainly occurred in spring and early summer. The highest detection rates of virus were in children with Non?severe community acquired pneumonia. The detection rates of children infection with HMPV and HBOV cause asthmatic bronchitis was higher than that infection with ADV. In our study,the infection rate of ADV、HMPV and HBOV were 63.84%、76.28% and 86.98% in patients ≤2 years old. Conclusion In Guangzhou,our study suggested that ADV and HBOV had been important agents causing ARTIs in pediatric patients.HMPV infection rates were relatively low.HMPV and HBOV are the most important pathogens in infants and toddlers with wheezing.