摘要目的 探讨白细胞介素9(IL?9)在晚期胃癌的作用.方法 选取本院2017年1月至2017年12月治疗的晚期胃癌患者30例和健康志愿者15例,使用ELISA方法检测健康志愿者和晚期胃癌患者外周血IL?9的浓度.通过IL?9在体外与胃癌细胞株共培养,使用流式细胞仪检测胃癌细胞的凋亡比例以及增殖抗原Ki?67的比例变化.结果 健康人外周血IL?9浓度高于晚期胃癌患者(P<0.05),而经过治疗后患者IL?9水平明显改善(P<0.05).体外培养显示IL?9实验组胃癌细胞凋亡比例增加[(37.5±5.8)%比(23.3±6.5)%,P<0.05],Ki?67阳性细胞比例也低于对照组[(76.2±5.1)%比(86.9± 3.7)%,P<0.05].结论 IL?9与机体的抗肿瘤免疫相关,在体外可以促进胃癌细胞的凋亡,抑制肿瘤生长.

abstractsObjective To investigate the effect of interleukin?9(IL?9)in advanced gastric cancer. Methods Thirty patients with advanced gastric cancer admitted to our hospital between January 2017 and December 2017 and a contemporary cohort of 15 healthy volunteers was included in the study. The concentration of IL?9 in peripheral blood of healthy volunteers and patients with advanced gastric cancer was measured by ELISA. IL?9 was co?cultured with gastric cancer cell line in vitro. The changes in the proportions of gastric cancer cell apoptosis and proliferating antigen Ki?67 were determined by flow cytometry. Results The IL?9 level in peripheral blood of healthy subjects was significantly higher than that in patients with advanced gastric cancer(P<0.05),and the IL?9 level was significantly improved in the patients after the treatment (P<0.05). In vitro culture showed that the proportion of gastric cancer cell apoptosis in the IL?9 study group increased,whereas the percentage of apoptosis cells was significantly higher than that in the control group[(37.5±5.8)% vs(23.3±6.5)%,P<0.05],and the percentage of Ki?67 positive cell was also lower than that in the control group [(76.2 ± 5.1)% vs(86.9 ± 3.7)% ,P<0.05]. Conclusion IL?9 is related to the body’s antitumor immunity,which may promote gastric cancer cell apoptosis,and inhibit tumor growth in vitro.