摘要目的 探究陈旧性心肌梗死(OMI)伴心力衰竭患者的单核细胞差异表达基因及相关信号通路.方法 通过GEO数据库获取GSE59867基因芯片数据集,筛选伴心衰的陈旧性心肌梗死患者作为心衰组(HF组),稳定冠心病组作为对照组,无伴心衰的OMI患者作为非心衰组(non-HF组),采用GEO2R工具筛选差异表达基因,并采用DAVID和STRING分析平台进行GO基因本体分析、KEGG信号通路分析及蛋白质相互作用网络分析(PPI分析).结果 与对照组相比,HF组外周血单核细胞基因表达以下调为主,共筛选出16个下调基因;与non-HF组相比,HF组共筛选出14个下调基因.分别分析其枢纽基因,发现两组枢纽基因高度重叠,包括HBA1、HBA2、HBB、HBE1、HBG1、HBG2、HBD、AHSP、HP、HBZ、CA1.枢纽基因主要涉及血红蛋白表达、氧气输送、过氧化物分解、抗疟疾、抗非洲锥虫病等生物学过程.结论 对于陈旧性心肌梗死伴心衰的患者,外周血单核细胞中血红蛋白家族基因的低表达可能是潜在的重要研究及治疗靶点.

abstractsObjective To investigate the differentially expressed genes and related signaling pathways in monocytes of patients with old myocardial infarction (OMI) and heart failure (HF).Methods The GSE59867 gene-chip dataset was obtained from the GEO database.Based on dataset screening,patients with OMI and HF were included as the HF group,those with stable coronary heart disease as the control group,and the OMI patients without HF were included as non-HF group.The differentially expressed genes were screened by GEO2R tool.The DAVID and STRING analysis platforms were used to perform gene ontology (GO) analysis,KEGG pathway analysis and protein-protein interaction (PPI) network analysis.Results Compared with the control group,the gene expression levels in peripheral blood monocytes in HF group was mainly down-regulated,and 16 down-regulated genes were screened.Compared with the non-HF group,14 down-regulated genes were screened in the HF group.The hub genes in each group were determined,respectively,and were found to be highly overlapping in the two groups,including HBA1,HBA2,HBB,HBE1,HBG1,HBG2,HBD,AHSP,HP,HBZ,and CA1.The hub genes were mainly involved in biological processes covering hemoglobin expression,oxygen transport,peroxide decomposition,and resistance to malaria and African trypanosomiasis.Conclusion Low expression of hemoglobin family genes in peripheral blood monocytes may be a potential research and therapeutic target for patients with OMI and HF.