摘要目的:探讨微RNA（miR）-34b/c rs4938723基因多态性与肾母细胞瘤易感性的相关性。方法:选取从2014年3月至2018年3月在本院确诊的133例肾母细胞瘤患者，作为病例组，在本院征召的志愿者中随机选取529与对照组性别、年龄等无统计学差异的健康人群作为对照组，提取他们血液DNA，采用Taq-man荧光定量PCR技术，对miR-34b/c的rs4938723进行检测。统计不同基因型在观察组和对照组中的频率，并分析不同的基因型与肾母细胞瘤易感性的相关性。结果:病例组和对照组均得到了较好的基因分型结果。病例组中，rs4938723位点的三种基因型TT、TC和CC分布频率与对照组差异无统计学意义（ P>0.05）。2组的显性模型（TC+CC/TT），隐性模型（TT+TC/CC）和加性模型（TT/TC/CC）的差异均无统计学意义（ P>0.05）。不同性别中miR-34b/c rs4938723的基因型频率分布与肾母细胞瘤发生差异无统计学意义（ P>0.05）。在总人群和男女性人群中miR-34b/c rs4938723等位基因频率分布差异无统计学意义（ P>0.05），miR-34b/c rs4938723等位基因频率分布与肾母细胞瘤无关（ P>0.05）。病例组中rs4938723三种基因分型的病理分级，Ⅰ级、Ⅱ级、Ⅲ级和Ⅳ级的差异均无统计学意义（ P>0.05）。 结论:miR-34b/c rs4938723多态性位点与肾母细胞瘤的遗传易感性无关。

abstractsObjective:To investigate the association between miR-34b/c rs4938723 gene polymorphism and susceptibility to Wilms’ tumor.Methods:A total of 133 patients with a confirmed diagnosis of Wilms’ tumor in our hospital between March 2014 and March 2018 were enrolled as the case group. Among volunteers recruited in our hospital, 529 gender- and age-matched healthy subjects were randomly enrolled as the control group. Of all subjects, blood DNA as extracted; Taq-man quantitative PCR was used to determine the miR-34b/c rs4938723. The frequency of genotypes in the case and control groups was counted. The association between genotypes and susceptibility to Wilms’ tumor was analyzed.Results:The genotypes in the case group and the control group were successfully obtained. In the case group, the distribution frequencies of the genotypes TT, TC, and CC at rs4938723 were not statistically different from those in the control group ( P>0.05) . The two groups also did not differ significantly in the dominant model (TC+CC/TT) , recessive model (TT+TC/CC) and additive model (TT/TC/CC) (all P>0.05) . In either gender, the distribution frequencies of miR-34b/c rs4938723 genotypes were not statistically associated with development of Wilms’ tumor ( P>0.05) . The distribution frequencies of miR-34b/c rs4938723 allele did not differ statistically in the entire study population or in either gender ( P>0.05) , and was not related to development of Wilms’ tumor ( P>0.05) . In the case group, the rs4938723 genotypes did not differ statistically among pathological grades Ⅰ, Ⅱ, Ⅲ and Ⅳ ( P>0.05) . Conclusion:miR-34b/c rs4938723 polymorphism is not associated with genetic susceptibility to Wilms’ tumor.