摘要目的:探讨神经生长因子（NGF）对牙周炎患者牙龈组织内炎症反应和牙槽骨密度的影响。方法:选择2016年3月至2019年3月山西省人民医院就诊的牙周炎患者80例。随机分为常规治疗组（40例，龈上洁治，龈下刮治术等常规治疗）和NGF组（40例，龈上洁治，龈下刮治术等常规治疗的同时，每天在牙龈周围涂抹鼠NGF），另选取牙周组织正常的40例健康志愿者作为对照组。免疫组织化学染色检测CD3 + T细胞、CD4 + T细胞、CD68 +巨噬细胞和CD20 + B细胞数目；三维锥形束CT机检测牙槽骨密度。 结果:对照组牙龈组织内CD68 +巨噬细胞、CD3 + T细胞、CD4 + T细胞和CD20 + B细胞数目分别为（3.2±0.4、（1.6±0.2）、（0.9±0.1）和（0.4±0.1）个/高倍视野（HPF），常规治疗组分别为（29.5±4.9）、（69.3±8.2）、（28.2±3.9）和（12.6±2.2）个/HPF，均 P<0.001, NGF组牙龈组织内CD68 +巨噬细胞、CD3 + T细胞和CD4 + T细胞数目为（16.6±5.6）、（26.1±3.4）、（8.6±0.9）个/HPF，相对于常规治疗组，差异均具有统计学意义（均 P<0.001）；对照组牙槽骨密度为（386.4±14.7）g/cm 2，常规治疗组为（329.3±11.2）g/cm 2，两组之间差异有统计学意义（ P<0.001）；NGF组牙槽骨密度为（361.1±15.0）g/cm 2，相对于常规治疗组，差异有统计学意义（ P=0.007）。 结论:NGF能够抑制牙周炎患者牙龈组织内巨噬细胞、T细胞和CD4 + T细胞浸润，增加牙槽骨密度。

abstractsObjective:To investigate the effect of nerve growth factor (NGF) on the inflammatory response in the gingival tissue and alveolar bone mineral density of patients with periodontitis.Methods:Included in this study were 80 patients with periodontitis who visited Shanxi Provincial People’s Hospital between March 2016 and March 2019. The patients were randomly divided into the conventional treatment group ( n=40) to receive routine care such as supragingival cleansing and subgingival curettage, and the NGF group ( n=40) to receive daily application of mouse NGF in addition to the routine care such as supragingival cleansing and subgingival curettage. Another 40 healthy volunteers with normal periodontal tissues were recruited as the control group. Immunohistochemical staining was used to examine the number of CD3 +, CD4 + T cells, CD68 + macrophages, and CD20 + B cells. Three-dimensional cone-beam CT was used to measure the alveolar bone mineral density. Results:The numbers of CD68 + macrophages, CD3 +, CD4 + T cells and CD20 + B cells in the gingival tissue were 3.2±0.4, 1.6±0.2, 0.9±0.1 and 0.4±0.1 per high power field (HPF) respectively in the control group, and 29.5±4.9, 69.3±8.2, 28.2±3.9 and 12.6±2.2 cells per HPF (all P<0.001) respectively in the conventional treatment group. The numbers of CD68 + macrophages, CD3 + and CD4 + T cells were 16.6±5.6, 26.1±3.4, and 8.6±0.9 per HPF, with statistically significant differences compared with the conventional treatment group ( P<0.001) . The alveolar bone mineral density (386.4±14.7) g/cm 2 in the control group vs (329.3±11.2) g/cm 2 in the conventional treatment group, with significant difference between the two groups ( P<0.001) . The alveolar bone mineral density in the NGF group was (361.1±15.0) g/cm 2, with which was significantly different compared with the conventional treatment group ( P=0.007) . Conclusion:Among patients with periodontitis, use of NGF can inhibit the infiltration of macrophages, T cells and CD4 + T cells in the gingival tissue, and increase the alveolar bone mineral density.