摘要探讨舒芬太尼联合miR-410-3p调控膀胱癌细胞增殖、凋亡、迁移及侵袭的分子机制。体外培养人膀胱癌细胞RT4，分别加入不同剂量的舒芬太尼处理细胞，分别将anti-miR-NC、anti-miR-410-3p转染至RT4细胞，分别将anti-miR-NC、anti-miR-410-3p转染至RT4细胞后加入舒芬太尼处理。研究发现舒芬太尼可通过上调miR-410-3p的表达从而减弱膀胱癌细胞增殖、迁移及侵袭能力，诱导细胞凋亡。

abstractsThe purpose of this study was to investigate the molecular mechanism by which sufentanil interferes with the proliferation, apoptosis, migration and invasion of bladder cancer cells through c regulating miR-410-3p. Human bladder cancer cell RT4 cultured in vitro were added with different doses of sufentanil. Then, the RT4 cells were transfected with nti-miR-NC and anti-miR-410-3p. After the transfection, some of the RT4 cells were treated with sufentanil, while some were not. Our study showed that sufentanil could reduce the proliferation, migration, and induce apoptosis of bladder cancer cells by up-regulating the miR-410-3p expression.