摘要目的:建立小鼠脑血管网络的近红外二区光声成像系统，并探究光声波开启血脑屏障的可行性。方法:使用1064 nm波长激光对不同浓度的纳米材料PATQ-T进行光声成像。暴露3~8周龄KM小鼠颅骨，通过尾静脉注射将纳米材料打进小鼠体内，在1064 nm的波长下对进行注射前后的脑血管网络进行成像，然后用532 nm的激光产生光声波，观察血脑屏障在光声波作用下的开启情况。结果:近红外光声成像系统拥有较好横向分辨率，约为80 μm。通过光声成像可以看到PATQ-T的光声信号强度随浓度的增加而增强。在1064 nm的波长下，PATQ-T显示了较好的光声增强效果。经光声波激发后，在血管网络周围出现了明显的光声信号增强。结论:通过光声成像技术可以对小鼠脑血管网络进行成像。光声波具有开启血脑屏障的作用。

abstractsObjective:To establish a near-infrared second-window (NIR-II) photoacoustic imaging system for visualizing the cerebrovasculature in mice, and to investigate whether photoacoustic waves could be used to open the blood-brain barrier.Methods:Firstly, photoacoustic imaging of the nano-material PATQ-T at different concentrations was completed by using a 1064 nm laser. A cranial fenestration was then performed in KM mice aged 3-8 weeks. Nanomaterials were injected into the mice via caudal vein. Photoacoustic imaging of the mouse cerebrovasculature with the 1064 nm laser was conducted before and after the injection. A 532 nm laser was used to generate the photoacoustic waves attempted for photoacoustic opening of the blood-brain barrier.Results:The NIR-II photoacoustic imaging system showed a high lateral resolution of approximately 80 μm. On this system, the photoacoustic signal intensity of PATQ-T increased along its concentration. Favorable enhancement of PATQ-T was achieved by using the 1064 nm laser. Photoacoustic wave excitation led to significant enhancement of photoacoustic signals around the cerebrovasculature.Conclusion:Photoacoustic imaging allows visualization of cerebrovasculature in mice. Photoacoustic waves are useful to open the blood-brain barrier.