摘要目的:设计并制备以明胶为针尖、透明质酸为底座的可分离式微针，探讨其实现透皮载药的可行性。方法:采用模板法制备微针，选用明胶作为载药针尖，透明质酸作为基座。扫描电子显微镜观察微针微观形貌，万能拉力机检测其力学性能，穿刺实验证明其结构可分离。结果:制备的微针形貌完整，呈圆锥形针状阵列；力学测试结果表明，当微针应变为300 μm时，应力为（25.72±8.07）N；透皮实验结果表明，明胶微针可穿透新西兰大白兔耳朵，透明质酸底座被少量清水溶解。结论:可分离式微针制作简单，使用方便，可穿透皮肤角质层，具有潜在的透皮载药应用前景。

abstractsObjective:To design and prepare a separable microneedle which employs gelatin as the tip and hyaluronic acid as the base, and to verify the feasibility of transdermal drug delivery using the prepared microneedle.Methods:The microneedles were prepared by template method with gelatin as the drug-loading tip and hyaluronic acid as the base. The morphology of the microneedles were observed by scanning electron microscopy. The mechanical properties of microneedle were tested by universal tension machine. The structural separability of the microneedles was verified by puncture experiments.Results:The prepared microneedles were structurally integrated in form of conical needle arrays. The mechanical test showed a stress of (25.7164±8.0690) N with a strain of 300 μm upon the microneedle. In puncture experiment, the tip of microneedle was able to penetrate the ear of New Zealand rabbits while the base was dissoluble in a small amount of water.Conclusion:The designed microneedle in this study is structurally simple, easy to use, competent to penetrate the cuticle layer, and therefore is potentially useful for transdermal drug delivery.