摘要目的:构建金黄色葡萄球菌USA300 JE2 sfa和 sbn基因敲除株并研究铁载体相关基因对金黄色葡萄球菌生存的影响。 方法:以pIMAY质粒为载体，构建重组质粒pIMAY-? sfa、pIMAY-? sbn，电转入金黄色葡萄球菌USA300 JE2，利用同源重组技术分别构建金黄色葡萄球菌USA300 JE2 ? sfa、? sbn及? sfa? sbn基因敲除株，并对基因敲除株进行生长曲线测定。 结果:通过构建含同源片段的重组质粒，成功敲除 sfa基因、 sbn基因及 sfa和sbn基因，3种敲除株在缺铁条件下的生存能力有不同程度的下降。 结论:sfa基因和 sbn基因敲除对铁限制环境下金黄色葡萄球菌生存有影响。

abstractsObjective:To construct the sfa and/or sbn gene deletion strains of Staphylococcus aureus USA300 JE2, and to investigate the effect of siderophore-related genes on viability of S. aureus. Methods:pIMAY plasmid was used as the vector for construction of the recombinant plasmids pIMAY-? sfa and pIMAY-? sbn which were subsequently electrotransformed into the S. aureus strain USA300 JE2. Homologous recombination techniques were used to construct the S. aureus USA300JE2 strains with ? sfa, and/or ? sbn knockout. The growth curve of gene knockout strains were measured. Results:By constructing recombinant plasmids containing homologous fragments, gene knockout of sfa and/or sbn were successful. The three knockout strains showed varied decline in viability under iron-deficient conditions. Conclusion:In iron-limited environments, knockout of sfa and/or sbn gene may affect the viability of Staphylococcus aureus.