摘要目的 观察正常大鼠发育过程中暗适应视网膜电图(ERG)a波的变化特点.方法 实验研究.采用RETI-scan系统,环形角膜电极和不锈钢针状电极,对8组不同日龄大鼠(21、25、32、35、37、46、60及90 d)记录不同刺激光强度下(0.03、0.95、3和9.5 cd·s·m-2)的暗适应ERG.导出数据,先采用Matlab 7.4对数据进行预处理,然后根据数学模型使用Excel提供的规划求解模型拟合a波并分析模型中的2个参数:饱和波幅( Rmp3)与敏感度(S).结果 不同刺激光强度下Rmp3的最小值均出现在25日龄大鼠,光强为0.03 cd·s·m-2时其最大值出现在60日龄大鼠,余则出现在46日龄大鼠.8组大鼠的S均与刺激光强度呈线性关系(S=ks·I+bs),除60日龄大鼠外,曲线的斜率ks基本保持不变,约为0.09,日龄主要影响常量bs.结论 正常大鼠ERG a波的饱和波幅及敏感度均不同程度地受日龄的影响.

abstractsObjective To investigate the characteristics of the dark-adapted electroretinography (ERG) a-wave during development in normal rats.Methods Inthis experimental study,the dark-adapted ERG of eight groups of Rcs-rdy+-p+rats at different ages (21,25,32,35,37,46,60 and 90 d) were recorded by a series of flashes (0.03,0.95,3 and 9.5 cd·s·m-2) using a RETI-scan system and gold-foil ring form corneal recording electrode and a stainless steel needle electrode custom made in the lab.Data were exported to the computer and processing by a Matlab 7.4 software,then were modeled with the solver model of Excel and two parameters of the a-wave were analyzed: the saturation a-wave amplitude (Rmp3) and sensitivity (S).Results The minimum Rmp3 appeared at 25 days old group.When the intensity of light was 0.03 cd·s· m-2,the maximum of Rmp3 appeared at 60 days old group,under other intensity of light,it appeared at 46 days old group.It showed that there was a linear relationship between the sensitivity of the 8 groups and intensity of the light (S=ks·I+bs).Excluding the 60 days old group,the effects of age was mainly on the constant-bs but not the slope of the curve-ks,ks was equal to 0.09.Conclusion The Rmp3 and sensitivity of a-wave of the ERG can be affected by age with vary degrees.