摘要目的 观察温和高温下胰腺癌细胞株PANC1对吉西他滨敏感性的影响.方法 应用1 μmol/L吉西他滨处理胰腺癌PANC1细胞1h后,分别置37、42、45℃水浴锅孵育1h,再继续培养0、24、48 h.采用CCK-8法检测细胞增殖,AV/PI染色后上流式细胞仪检测细胞凋亡率.结果 42℃及45℃孵育细胞24、48 h后的细胞增殖均较37℃孵育的细胞显著降低(0.96±0.05、0.88±0.03比1.05±0.02;1.28±0.04、0.94±0.04比1.49±0.09；t值分别为4.367、25.120、3.510、12.101,P值均＜0.05),且45℃孵育的细胞增殖显著低于42℃孵育的细胞(t值分别为3.348、11.732,P值均＜0.05).37、42、45℃孵育的吉西他滨处理的PANC1细胞48 h后的细胞凋亡率分别为(7.125 ±0.064)％、(9.985±0.615)％、( 14.845±1.987)％,3组间的差异均具有统计学意义(t值分别为10.320、9.832、4.575,P值均＜0.05).结论 温和高温可以增加PANC1细胞对吉西他滨的敏感性.

abstractsObjective To investigate the effect of mild hyperthermia on chemoresistance of gemcitabine in the pancreatic cancer cell line PANC1.Methods The PANC1 cell was treated with gemcitabine ( 1 μmol/L) for 1 h,then was heated at 37℃,42℃ and 45℃ for 1 hour,and was cultured for 0 h,24 h,48 h.Cell growth was analyzed by CCK-8 assay.Cell apoptosis was analyzed by Annexin Vfluorescein isothiocyanate ( FITC)/propidium iodide (PI) staining.Results The proliferation of cells at 42℃ and 45℃ for 24 h and 48 h were significantly lower than that at 37℃ (0.96 ± 0.05,0.88 ± 0.03vs 1.05 ±0.02;1.28 ±0.04,0.94 ±0.04vs 1.49 ±0.09;t =4.367,25.120,P ＜0.05).The proliferation of cells at 45℃ was significantly lower than that at 42℃ (t =3.348,11.732,P ＜0.05).The cell apoptosis rates at 37℃,42℃,45℃ after 48 h were (7.125 ±0.064)％,(9.985 ±0.615)％,(14.845 ± 1.987)％,the difference among the 3 groups was statistically significant (t =10.320,9.832,4.575,P ＜0.05).Conclusions Mild hyperthermia can reduce chemoresistance of gencitabine in pancreatic cancer cell PANC1.