摘要目的 观察沉默S100A4基因表达对人胰腺癌BxPC-3、AsPC-1细胞肿瘤相关基因COX-2、bcl-2 、Surviving、MMP-9 mRNA表达的影响,探讨它们之间的关系.方法 应用靶向S100A4的小干扰RNA(siRNA)转染人胰腺癌BxPC-3、AsPC-I细胞,应用无同源性的siRNA-C转染细胞作为阴性对照,以未转染细胞作为对照组.采用RT-PCR检测干扰后细胞S100A4、COX-2、Survivin、MMP-9、bcl-2 mRNA的表达.结果 人胰腺癌BxPC-3细胞的对照组、siRNA-C组、siRNA-S100A4组的S100A mRNA表达量分别为0.661 ±0.023、0.659±0.043、0.379±0.039；COX-2 mRNA表达量分别为0.760±0.026、0.830±0.017、0.443±0.006；Survivin mRNA表达量分别为0.948±0.049、0.909±0.081、0.068 ±0.006;bcl-2mRNA表达量分别为0.462±0.018、0.421±0.049、0.184±0.025；MMP-9 mRNA表达量分别为0.813±0.008、0.908±0.063、0.246±0.027.AsPC-I细胞的对照组、siRNA-C组、siRNA-S100A4组的S100A4mRNA表达量分别为0.641±0.042、0.626±0.053、0.320±0.081;COX-2 mRNA表达量分别为0.727±0.021、0.743±0.025、0.560±0.035；Survivin mRNA表达量分别为0.994±0.032、0.984±0.049、0.063±0.005;bcl-2 mRNA表达量分别为0.458±0.004、0.537±0.046、0.181±0.007;MMP-9 mRNA表达量分别为0.698±0.011、0.718±0.073、0.199±0.013.2株细胞的siRNA-S100A4组的S100A、COX-2、Survivin、bcl-2、MMP-9 mRNA表达量均较其相应的siRNA-C组及对照组显著减少(P值均＜0.01),而siRNA-C组与对照组间的表达量差异无统计学意义.结论 S100A4通过上调COX-2、Survivin、bcl-2、MMP-9基因的表达在胰腺癌发生、发展中发挥作用.

abstractsObjective To investigate the effect of S100A4 silencing on tumor related gene COX-2,bcl-2,Surviving,MMP-9 mRNA expressions of pancreatic cancer BxPC-3,AsPC-1 cells,and explore their relationship.Methods Small interfering RNA interfering S100A4 gene (siRNA-S100A4) was applied to transfect human pancreatic cancer BxPC-3,AsPC-1 cells,and nonhomologous siRNA-C was used as negative control,and cells without transfection were used as control group.The expressions of S100A4,COX-2,Survivin,MMP-9,bcl-2 mRNA after interference were detected by using RT-PCR.Results S100A mRNA expressions of BxPC-3's control group,siRNA-C group,siRNA-S100A4 group were 0.661 ± 0.023,0.659 ± 0.043,0.379 ± 0.039,and expressions of COX-2 mRNA were 0.760 ± 0.026,0.830 ± 0.017,0.443 ±0.006,and expressions of Survivin mRNA were 0.948 ± 0.049,0.909± 0.081,0.068 ± 0.006,and expressions of bcl-2 mRNA were 0.462 ±0.018,0.421 ±0.049,0.184 ±0.025,and expressions of MMP-9 mRNA were 0.813 ± 0.008,0.908 ± 0.063,0.246 ± 0.027.S100A mRNA expressions of AsPC-I's control group,siRNA-C group,siRNA-S100A4 group were 0.641 ± 0.042,0.626-± 0.053,0.320 ± 0.081,and expressions of COX-2 mRNA were 0.727 ± 0.021,0.743 ± 0.025,0.560 ± 0.035,and expressions of Survivin mRNA were 0.994 ± 0.032,0.984 ± 0.049,0.063 ± 0.005,and expressions of bcl-2 mRNA were 0.458 ±0.004,0.537 ± 0.046,0.181 ± 0.007; and expressions of MMP-9 mRNA were 0.698 ± 0.011,0.718 ± 0.073,0.199± 0.013.The expressions of S100A,COX-2,Survivin,bcl-2,MMP-9 mRNA in groups with siRNA-S100A4 transfection were significantly lower than those of siRNA-C group and control group (P ＜0.01),but the difference between siRNA-C group and control group was not statistically significant.Conclusions S100A4 plays a role in the pathogenesis of pancreatic cancer through up-regulation of COX-2,Survivin,bcl-2,MMP-9 expressions.