摘要目的：验证氧化苦参碱（ OM ）在体外对胰腺星状细胞（ PSC ）激活的抑制作用，探讨其作用机制。方法观察经不同浓度OM干预后大鼠PSC细胞株LTC-14在转化生长因子β1（ TGF-β1）诱导激活后的增殖能力，应用ELISA法检测细胞超氧化物歧化酶（ SOD）水平，用实时定量PCR法检测细胞丝裂原激活蛋白激酶p38（p38-MAPK） mRNA表达量。结果对照组及0．1、0．5、1、2、5 g／L OM干预30 min后再加8μg／L TGF-β1诱导组（ OM组）细胞在培养12 h后的细胞增殖分别为1．51±0．08、1．50±0．07、1．15±0．04、1．15±0．04、1．08±0．06、1．08±0．10，0．5 g／L以上的OM组低于对照组，差异有统计学意义（P值均为0．000）。对照组、TGF-β1组及0．5、1 g／L OM组培养12 h后LTC-14细胞SOD的水平分别为0．087±0．005、0．073±0．004、0．085±0．010、0．086±0．007，各组间差异无统计学意义；p38-MAPK mRNA表达量分别为1．000±0．000、1．979±0．505、0．606±0．111、0．303±0．159，TGF-β1组显著高于对照组（P＝0．002），两个OM组均低于TGF-β1组（P值均为0．000），但两个OM组间差异无统计学意义（P＝0．208）。结论 OM体外能够抑制PSC的激活，其作用机制可能是通过抑制p38-MAPK mRNA表达实现的。

abstractsObjective To clarify whether oxymatrine ( OM) could suppress the activation of pancreatic stellate cells ( PSC) and explore the potential molecular mechanism .Methods The proliferation of PSC line LTC 14 being activated by TGF-β1 with OM treatment at different concentrations (OM group) was measured. SOD level was determined by ELISA and p 38-MAPK mRNA was determined by real-time PCR.Results The proliferation of PSC in the control group , 0.1, 0.5, 1, 2, 5 g/L OM group was (1.51 ±0.08), (1.50 ± 0.07), (1.15 ±0.04), (1.15 ±0.04), (1.08 ±0.06), and (1.08 ±0.10), respectively.The level of the control group was lower than the groups where the concentration of OM reached or exceeded 0.5mg/ml ( all P=0.000).SOD level of LTC 14 cells in the control group, TGF-β1 group, 0.5 and 1 g/L OM group was (0.087 ±0.005), (0.073 ± 0.004), (0.085 ± 0.010), and (0.086 ± 0.007), respectively. No statistically significant difference existed among the groups (P=0.095).The p38-MAPK mRNA expression of PSC in the control group, TGF-β1 group, 0.5, and 1 g/L OM group was (1.000 ±0.000), (1.979 ± 0.505), (0.606 ±0.111), and (0.303 ±0.159), respectively.The p38-MAPK mRNA level of TGF-β1 group was higher than that of the control group (P=0.002), and that of 0.5 mg/ml OM group and 1 mg/ml OM group was lower that of TGF-β1 group ( P=0.000 ) , while no statistical difference was found between 0.5 mg/ml OM group and 1 mg/ml OM group.Conclusions OM could suppress the activation of PSC in vitro and the suppression of p38-MAPK mRNA expression may be involved .