摘要目的：探讨给予外源性硫化氢（ H2 S）及其合成酶抑制剂炔丙基甘氨酸（ PAG）对雨蛙素诱导的小鼠急性胰腺炎（ AP）自噬功能的影响。方法60只雄性BALB／c小鼠，按数字表法随机分为对照组、AP组、硫氢化钠（ NaHS）组、PAG组。采用连续6次腹腔注射雨蛙素（50μg／kg体重）、每次间隔1 h的方法建立小鼠AP模型。 NaHS组及PAG组在造模前1 h分别腹腔注射NaHS 10 mg／kg体重、PAG 50 mg／kg体重，对照组及AP组小鼠腹腔注射等容积生理盐水。于第1次注射雨蛙素12 h后处死小鼠，取血检测血清淀粉酶、脂肪酶含量，采用去蛋白分光光度法测定血清H2 S含量；取部分胰腺组织常规行病理学检查并进行病理评分；采用Real-time PCR法检测胰腺组织硫化氢合成酶胱硫醚-γ裂解酶（ CSE ） mRNA表达，蛋白质印迹法检测胰腺组织自噬相关的微管相关蛋白轻链3-Ⅱ（ LC3-Ⅱ）与LC3-Ⅰ的比值及泛素结合蛋白（ p62）的表达。结果对照组血清淀粉酶、脂肪酶、H2 S浓度及胰腺组织CSE mRNA、LC3-Ⅱ／LC3-Ⅰ值和p62表达量分别为（2700±100）U／L、（70±20）U／L、（22．9±1．7）mmol／L、1．0±0．1、0．419±0．080、0．227±0．140；AP组小鼠分别为（17290±500）U／L、（520±40）U／L、（31．3±3．0） mmol／L、5．4±0．4、1．184±0．120、1．985±0．210；NaHS组分别为（27784±1200）U／L、（900±80）U／L、（38．6±3．3） mmol／L、6．9±0．9、1．600±0．210、4．229±0．050；PAG 组分别为（13750±2000） U／L、（370±20）U／L、（24．5±2．1）mmol／L、4．2±0．5、0．745±0．130、1．203±0．080。 AP组上述指标均显著高于对照组，NaHS组又较AP组进一步显著升高，而PAG组较AP组显著降低，差异均有统计学意义（ P值均＜0．05）。 NaHS组胰腺病理损伤较AP组进一步加重，而PAG组胰腺病理损伤较AP组显著减轻。结论 PAG能够降低血清淀粉酶、脂肪酶水平，减轻AP时自噬功能的受损状况，减轻AP的严重程度。

abstractsObjective To investigate the effect of H 2 S and its synthetase inhibitor propargylglycine ( PAG) on the autophagic function in caerulein-induced acute pancreatitis ( AP) mice.Methods A total of 60 male BALB/c mice were randomly divided into control , AP, NaHS and PAG group using random number method.AP was induced in mice via hourly intraperitoneal injection of caerulein (50 μg/kg) continuously for 6 hours.NaHS and PAG group received NaHS (10 mg/kg) or PAG (50 mg/kg) 1 h before the AP induction . A equal volume of normal saline solution was injected in control group and AP group .All the mice were killed at 12 h after the first caerulein injection and blood sample was collected for the detection of serum amylase and lipase content.Deproteinization spectrometry was used to detect serum H 2 S content, and pancreatic tissue was pathological examined and scored . Real-time PCR detected mRNA expression of CSE , and the protein expression of LC3-Ⅱ/LC3 Ⅰand p62 was measured using Western blot .Results Serum amylase, lipase,&nbsp;H2S, CSE mRNA, LC3Ⅱ/LC3Ⅰand p62 were (2 700 ±100)U/L, (70 ±20)U/L,(22.9 ±1.7)mmol/L, 1.0 ±0.1,0.419 ±0.080, 0.227 ±0.140 in control group; (17 290 ±500)U/L,(520 ±40)U/L, (31.3 ± 3.0)mmol/L, 5.4 ±0.4, 1.184 ±0.120, 1.985 ±0.210 in AP group; (27 784 ±1 200)U/L, (900 ± 80)U/L,(38.6 ±3.3)mmol/L, 6.9 ±0.9,1.600 ±0.210, 4.229 ±0.050 in NaHS group; (13 750 ± 2 000)U/L,(370 ±20)U/L, (24.5 ±2.1)mmol/L, 4.2 ±0.5, 0.745 ±0.130, 1.203 ±0.080 in PAG group.All those biomarkers detected above in AP group significantly increased compared with control group , which were much lower than those in NaHS group , but higher than those in PAG group , and the differences were statistically significant (all P<0.05).Pancreatic histological damage in NaHS group was more severe than that in AP group , which in PAG group was less severe than that in AP group .Conclusions PAG could greatly decrease serum amylase and lipase level , and reduce the damage on autophagy and the severity of AP .