摘要目的 观察细梗香草皂苷(LC)对人胰腺癌细胞株BxPC-3的增殖抑制作用,探讨其作用机制.方法 应用2、4、8、16、32、64 μg/ml LC干预BxPC-3细胞48、72 h,以不加LC的细胞作为对照组,采用MTT法检测BxPC-3细胞存活率,绘制生长曲线,确定LC的50％抑制浓度(IC50).以IC50的LC浓度干预BxPC-3细胞(LC组),采用流式细胞仪检测细胞凋亡和细胞周期,采用蛋白质印迹法检测凋亡相关蛋白PARP、caspase-3的表达.结果 8～32 μg/ml LC呈浓度依赖性抑制BxPC-3细胞的存活率,以IC50的LC 15 μg,/ml干预48 h后能促进细胞凋亡[(17.3±0.31)％比(1.5±0.22)％],但不影响细胞周期;和对照组相比,LC组caspase-3蛋白表达上调(2207.2±92.0比149.1±10.2),PARP蛋白表达下调(36.1±4.8比1593.4±29.7),差异均有统计学意义(P值均＜0.05).结论 LC能抑制胰腺癌BxPC-3细胞存活率,其机制可能是通过上调caspase-3蛋白表达,分解PARP,从而诱导细胞凋亡.

abstractsObjective To investigate the effect of lysimachia capillipes(LC) on proliferation of human pancreatic cancer cell line BxPC-3 in vitro and explore the potential mechanism.Methods BxPC-3 cells were treated by LC in different concentrations of 2,4,8,16,32,64 μg/ml for 48 and 72 hours,respectively,using untreated cells as control.The survival rate of BxPC-3 cells was measured by MTT method.The half inhibition concentration (IC50) of LC was determined by drawing growth curve.BxPC-3 cells were treated by LC in the concentration of IC50 (LC group),and cell apoptosis and cell cycle were examined by using flow cytometry.The protein expression of PARP and capase-3 was detected by Western blotting.Results LC in the concentration of 8-32 μg/ml inhibited the survival rate of BxPC-3 cells in a dose-dependent manner.After exposure to 15 μg/ml LC for 48 h,the apoptosis rate of BxPC-3 cells was increased [(17.3 ± 0.31)％ vs (1.5 ± 0.22) ％],but the cell cycle was not affected.The expression of caspase-3 protein was up-regulated [(2207.2 ± 92.0) vs 149.1 ± 10.2] and PARP protein was down-regulated [(36.1 ± 4.8) vs 1593.4 ±29.7] than control group,and the differences were statistically different (all P value ＜ 0.05).Conclusions LC can inhibit the growth of BxPC-3 cells,and the potential mechanism was associated with the induction of cell apoptosis by LC via upregulating caspase-3 protein expression and decompsing PARP protein.