摘要目的 观察红景天苷对人胰腺癌BxPC-3细胞裸鼠移植瘤的作用.方法 建立BxPC-3裸鼠皮下移植瘤模型,成瘤后按数字表法随机分为对照组、红景天苷25 mg组和50 mg组,每组6只.红景天苷组分别采用25、50mg·kg-1 ·d-1红景天苷灌胃给药,3周后取瘤称重,测量直径,Tunel细胞凋亡染色法检测各组移植瘤细胞凋亡情况.结果 对照组、红景天苷25 mg组和50 mg组移植瘤的重量分别为(1.561±0.416)、(0.742±0.272)、(0.276±0.064)g;体积分别为(2354.35±523.11)、(991.43±348.91)、(403.60±130.98)mm3;细胞凋亡率分别为(23.74±5.88)％、(49.30±6.75)％和(64.97±6.99)％,3组间差异均有统计学意义(P值均＜0.01).红景天苷呈剂量依赖性抑制移植瘤细胞增殖及促进移植瘤细胞凋亡.结论 红景天苷可能通过诱导胰腺癌BxPC-3细胞凋亡,进而抑制胰腺癌细胞增殖.

abstractsObjective To investigate the effect of salidroside on xenograft tumor of human pancreatic cancer cells BxPC-3 in nude mice.Methods BxPC-3 subcutaneous xenograft tumor nude mice model was established,and the mice were randomly divided into control group,salidroside 25 mg group and salidroside 50 mg group using random number method (6 mice in each group).25 mg/kg and 50 mg/kg salidroside by intragastric administration was performed per day for 3 weeks and the tumor was resected.The tumor was weighed and the diameter was measured.Cell apoptosis was detected by Tunel staining.Results As result,tumor weight was (1.561 ± 0.416)g in the control group,(0.742 ± 0.272)g in the 25 mg group and (0.276 ± 0.064) g in the 50 mg group respectively;tumor volume was (2354.35 ± 523.11) mm3,(991.43 ± 348.91) mm3 and (403.60 ± 130.98) mm3,respectively;and the apoptosis rate of the transplanted tumor cells was (23.74 ± 5.88) ％,(49.30 ± 6.75) ％ and (64.97 ± 6.99) ％,respectively,with significant difference among three groups (all P ＜ 0.01).The effect of salidroside on inhibiting proliferation and promoting apoptosis was dose dependent.Conclusions Salidroside can significantly inhibit the proliferation and growth of pancreatic cancer BxPC-3 cells via promoting cell apoptosis and inhibiting cell proliferation.