摘要目的研究日本血吸虫重组BCG-Sj26GST疫苗对小鼠脾细胞产生TNF-α和IL-1的影响。方法实验1分别采用10６CFU和10８CFU疫苗皮下免疫BALB/C鼠，免疫后8 w用日本血吸虫尾蚴进行攻击感染，感染后6 w剖杀小鼠，同时设有PBS对照组。实验2用106CFU疫苗皮下和静脉注射分别免疫小鼠，于免疫后0、4、8、10、14和16 w各剖杀4只，分离脾脏，用Sj26或丝裂原刺激脾细胞，用ELISA法检测脾细胞上清液中TNF-α水平，用成纤维细胞增殖法检测IL-1的生物活性。结果疫苗免疫，尾蚴攻击后，TNF-α和IL-1水平显著升高。动态观察发现皮下注射组TNF-α和IL-1于免疫后4～8 w达最高水平；静脉注射组TNF-α和IL-1分别于免疫后16 w和4～8 w达较高水平。结论日本血吸虫重组BCG-Sj26GST疫苗促进小鼠脾细胞较早分泌TNF-α和IL-1，它们在血吸虫保护性免疫中起重要作用。

abstractsObjective　To study the effects of recombinant BCG-Sj26GST vaccine of schistosoma japonicum of TNF-α and IL-1 produced by spleen cells of mice.Methods　In experiment 1,BALB/C mice were immnized subcutaneously by 106 and 108 CFU vaccine respectively,challenged with Sj cercariae on 8 w of immunization and killed on 6 w of infection to separate spleens,PBS served as control;in experiment 2,after immunized subcutaneously and intravenously by 106 CFU vaccine respectively,4 mice were killed to separate sleens on 0,4,8,10,14 and 16 w of immunization,spleen cells were stimulated by Sj26 or mitogens,the level of TNF-α in supernatant of spleen cells were detected by ELISA,that of IL-1 by fibroblast proliferative response.Results　Levels of TNF-α and IL-1 increased obviously by immunization with the vaccine against challenge with Sj cercariae;dynamic observation showed that TNF-α and IL-1 in the subcutaneous group reached the highest level on 4～8 w;that in the intravenous group on 16 w and 4～8 w respectively.Conclusions　Recombinant BCG-Sj26GST vaccine of schistosoma japonicum may promote spleen cell of mice to secrete TNF-α and IL-1 early,these cytokines may play an important role in protective immunity against schistosomiasis.