摘要目的 观察硒和维生素E(Vit E)对大鼠血清心肌酶和抗氧化能力的保护作用.方法 雄性Wistar大鼠80只,体质量70～90g.按体质量和2×2析因设计将大鼠分为4组:低硒低Vit E组(饲料中含低硒酵母23.42％,不含Vit E)；低硒常Vit E组(饲料中含低硒酵母23.42％、Vit E 160 mg/kg)；常硒低Vit E组(饲料中含低硒酵母46.84％,水中含亚硒酸钠0.25 mg/L,不含Vit E);常硒常Vit E组(饲料中含低硒酵母46.84％、Vit E 160 mg/kg,水中含亚硒酸钠0.25 mg/L),每组20只.大鼠饲用合成饲料,在喂养26周后腹腔麻醉,采集大鼠静脉血,制备血清,观察硒和Vit E对大鼠心肌酶和心肌抗氧化能力的影响及二者的交互作用.血清肌酸激酶(CK)测定采用连续监测法,肌酸激酶同工酶(CK-MB)和乳酸脱氢酶(LDH)测定采用免疫抑制法,全血谷胱甘肽过氧化物酶(GSH-Px)测定采用二硫代二硝基苯甲酸(DTNB)法,血清超氧化物歧化酶(SOD)测定采用黄嘌呤氧化酶法,总抗氧化能力(T-AOC)测定采用络合物比色法,丙二醛(MDA)测定采用硫代巴比妥酸比色法,活性氧(ROS)测定采用比色法.结果 大鼠血清CK、CK-MB、LDH和全血GSH-Px活力,血清T-AOC活力及MDA、ROS含量组间比较差异有统计学意义(F值分别为9.797、17.041、48.399,3.744、224.900、49.384、5.045,P均＜0.05).CK、CK-MB、LDH活力与常硒常Vit E组[(1056.80±250.98)、(721.70±129.98)、(404.65±72.49)U/L)]比较,2个低硒组[(1577.75±451.87)、(1239.15±344.99)、(884.25±133.84)U/L,(1474.21±398.38)、(1014.84±215.40)、(523.00±98.05)U/L]和常硒低Vit E组[(1180.10±245.51)、(948.75±173.68)、(676.70±193.63)U/L]明显升高(P均＜0.05)；MDA含量,与常硒常Vit E组[(3.010±1.270)× 103 nmol/L]比较,2个低硒组[(5.688±1.169)×103、(4.035±0.487)×103 nmol/L]明显升高(P均＜ 0.05)；GSH-Px活力,与2个常硒组[(96.611±8.238)×103、( 103.024±8.217)×103 U/L]比较,2个低硒组[(60.356±8.179)×103,(63.117±8.281)×103 U/L]明显降低(P均＜0.05).硒因素对CK、CK-MB和LDH活力有影响作用(F值分别为27.09、31.58、29.66,P均＜0.01),Vit E因素对CK-MB和LDH活力有影响作用(F值分别为18.9、11.2,P均＜0.0l),但二者对CK、CK-MB和LDH无交互作用(F值分别为0.02、0.00、2.22,P均＞0.05).硒因素对GSH-Px活力和MDA含量有影响作用(F值分别为6.74、95.68,P均＜0.05),Vit E因素对T-AOC活力,MDA和ROS含量有影响作用(F值分别为6.42、36.73、8.43,P均＜ 0.05),但二者仅对MDA有交互作用(F值为13.82,P ＜0.05).结论 长期硒或VitE缺乏,可以使机体的抗氧化能力降低,导致心肌损伤的发生,硒和Vit E可提高机体的抗氧化能力,起到保护心肌的作用.

abstractsObjective To observe protective effects on rat serum cardiac enzymes and the antioxidant capacity of selenium and vitamin E.Methods According to body weight and 2 × 2 factorial design,eighty male Wistas rats were randomly divided into four groups:low selenium and low vitamin E group(feed containing 23.42％ of the low selenium yeast,excluding vitamin E),low selenium and adequate vitamin E group (feed containing 23.42％ of the low selenium yeast and vitamin E 160 mg/kg),adequate selenium and low vitamin E group(feed containing 46.84％ of the low selenium yeast and sodium seleni 0.25 mg/L in water,excluding vitamin E),adequate selenium and adequate vitamin E group(feed containing 46.84％ of the low selenium yeast,vitamin E 160 mg/kg and sodium selenite 0.25 mg/L in water),20 rats every group.Rats were feed with synthetic feed,and given intraperitoneal anesthesia after 26 weeks of feeding.Blood was collected to observe the impact of selenium and vitamin E on rat cardiac enzymes and myocardial antioxidant capacity and their interactions.Serum creatine kinase (CK) was measured using the continuous monitoring method,creatine kinase isozymes (CK-MB) and lactate dehydrogenase(LDH ) using the immune suppression method,the whole blood GSH-Px assay using the dithiobis nitrohenzoic acid(DTNB) method,serum superoxide dismutase(SOD) using the xanthine oxidase method,total antioxidant capacity (T-AOC) using the complex colorimetry method,the content of propylene glycol (MDA) using the thiobarbituric acid colorimetric method,and reactive oxygen species(ROS) using the colorimetric method.Results Group differences of serum CK,CK-MB,LDH,whole blood GSH-Px activity,serum T-AOC vitality,MDA and ROS content were statistically significant(F=9.797,17.041,48.399,3.744,224.900,49.384,5.045,all P＜ 0.05).Compared with the two low selenium groups and one adequate selenium group,the vitalities of CK,CK-MB,LDH and the contents of MDA[(1577.75 ± 451.87),(1239.15 ± 344.99),(884.25 ± 133.84)U/L,(5.688 ±1.169) × 103 nmol/L; (1474.21 ± 398.38),(1014.84 ± 215.40),(523.00 ± 98.05)U/L,(4.035 ± 0.487 ) × 103 nmol/L and (1180.10 ± 245.51),(948.75 ± 173.68),(676.70 ± 193.63)U/L,(3.406 ± 0.146) × 103 nmol/L]increased significantly in adequate selenium and adequate vitamin E group[( 1056.80 ± 250.98),(721.70 ±129.98),(404.65 ± 72.49)U/L,(3.010 ± 1.270) × 103 nmol/L,all P ＜ 0.05) ].The activity of GSH-Px was obviously increased in the two adequate selenium groups[ (96.611 ± 8.238) × 103,(103.024 ± 8.217) × 103 U/L,all P ＜ 0.05],compared with the two low selenium groups[ (60.356 ± 8.179) × 103,(63.117 ± 8.281) × 103 U/L].Selenium affected the activities of CK,CK-MB and LDH(F =27.09,31.58,29.66,all P＜ 0.01 ),and vitamin E affected the activities of CK-MB and LDH(F=18.9,11.2.all P＜ 0.01 ),but both selenium and vitamin E had no interactions on the activities of CK,CK-MB and LDH (F=0.02,0.001,2.22,all P＞0.05).Selenium affected the activity of GSH-Px and the content of MDA(F=6.74,95.68,all P＜ 0.05),vitamin E affected the activity of T-AOC,the contents of MDA and ROS(F=6.42,36.73,8.43,all P＜0.05),but selenium and vitamin E had interactions only on the content of MDA(F =13.82,P＜ 0.05).Conclusions Long-term selenium or vitamin E deficiency,can reduce the body's antioxidant capacity,leading to the occurrence of myocardial injury.Selenium and vitamin E can improve the body's oxidation capacity,playing a role in myocardial protection.