摘要目的 对云南省鼠疫菌株进行基因分型,探究云南各型菌株之间的内在联系.方法 选取云南家鼠、野鼠及玉龙鼠疫菌共171株进行试验,采用鼠疫菌基因组23个差异片段(DFR)进行分型,并运用BioNumerics 5.0进行聚类分析.结果 23个差异片段将171株鼠疫菌分为7个基因组型:Genomovar5、Genomovar7、Genomovar9型及4个新发现的基因型.7株玉龙型菌株都为Genomovar5基因型;16株野鼠型(滇西纵谷型)鼠疫菌株被分为3个基因型,其中13株为Genomovar7基因型,2株为Genomovar9基因型,1株为新发现的Genomovar-yn1基因型;148株云南家鼠型(滇闽居民型)鼠疫菌株被分为4个基因型,其中145株为Genomovar9基因型,3株为3个新发现的基因型,分别为Genomovar-yn2、-yn3、-yn4基因型.生态型聚类分析显示,玉龙型鼠疫菌株与滇西纵古型(野鼠型)菌株的基因型较为相似(87.20％),与滇闽居民型(家鼠型)相似度较小(73.75％).2株滇西纵谷型菌株与滇闽居民型的主基因型同为Genomovar9基因型.滇西纵谷型Genomovar-yn1基因型与Genomovar7基因型相似,但缺失DFR11.滇闽居民型Genomovar-yn2、-yn3、-yn4基因型与Genomovar9基因型相似,但分别缺失了DFR10、DFR9、DFR 11.结论 在野鼠型鼠疫菌株中新发现了1种基因型,在家鼠型鼠疫菌株中新发现了3种基因型,在野鼠型鼠疫菌株中发现了家鼠型鼠疫菌株.玉龙鼠疫菌株的基因型与滇西纵谷型(野鼠型)菌株的基因型相似较高,与滇闽居民型(家鼠型)菌株的基因型相似度较小.

abstractsObjective To genotype Yersinia pestis and explore intrinsic relationship among different ecotypes of Yersinia pestis in Yunnan foci.Methods A total of 171 strains from three types of Yersinia pestis,house mouse,wild-type mouse and Yulong Yersinia pestis,were tested.Twenty-three different regions (DFR) were used to genotype and cluster analysis was performed using BioNumerics 5.0.Results A total of 171 Yersinia pestis were divided into 7 genotypes by 23 DFRs,which were Genomovar5,Genomovar7,Genomovar9 and 4 newly discovered genotypes.The genotypes of all Yulong plague were Genomovar5.The genotypes of the 16 strains of wild-type mouse plague (the highland of northwestern Yunnan Province type) were divided to 3 genotypes,13 of them were Genomovar 7,2 of them were Genomovar9,and 1 of them was newly discovered genotype Genomovaryn1.The genotypes of the 148 strains of house mouse plague(the residential area of Yunnan and Fujian Provinces type) were divided into 4 genotypes,145 of them were Genomovar9,and 3 of them were newly discovered including Genomovar-yn2,-yn3 and-yn4.The ecological typing results of clustering showed genotype of Yulong plague was similar to the highland of northwestern Yunnan Province type(wild-type mouse plague),and the percentage of similarity was up to 87.20％,but only up to 73.75％ to the residential area of Yunnan and Fujian Provinces type (house mouse plague).The genotypes of 2 wild-type strains of the highland of northwestern Yunnan Province type(wild-type mouse) and main genotypes of the residential area of Yunnan and Fujian Provinces type(house mouse)were Genomovar 9.The genotype of Genomovar-yn 1 of the highland of northwestern Yunnan Province type was similar to Genomovar 7,but lack of DFR 11.The genotypes of Genomovar-yn2,-yn3 and-yn4 of the residential area of Yunnan and Fujian Provinces type were similar to Genomovar 9,but lack of DFR 10,DFR 9 and DFR 11,respectively.Conclusions One newly genotype strain is found in wild-type mouse plague and 3 newly genotype strains are founded in house mouse plague.Wild-type mouse strains are founded in the house mouse strains.The similarity of genotype between Yulong plague and the highland of northwestern Yunnan Province type (wild-type mouse plague) is high while the similarity between Yulong plague and the residential area of Yunnan and Fujian Provinces type(house mouse plague) is low.