摘要目的 探讨慢性血吸虫病患者结肠腺咬合蛋白(occludin)与紧密连接蛋白-1 (ZO-1)的表达及其临床意义.方法 选取50例慢性血吸虫病患者及9例正常结肠黏膜标本,采用免疫组织化学(SABC)染色法,检测occludin与ZO-1在结肠腺上皮细胞的表达情况.结果 与对照组比较,慢性血吸虫病组结肠腺结构不完整、腺体萎缩;慢性血吸虫病组结肠腺上皮细胞occludin与ZO-1阳性颗粒平均灰度值(159.26±6.57、166.07±5.44)明显高于对照组(155.53±5.90、162.16±5.14,P＜0.05),阳性单位值(37.27±2.63、34.69±2.19)明显低于对照组(38.85±2.42、36.37±2.11,P＜0.05);慢性血吸虫病组结肠腺occludin与ZO-1表达量的减少与性别及年龄无关(P＞0.05).结论 血吸虫感染可以损伤结肠腺上皮细胞间的紧密连接蛋白,从而破坏患者结肠黏膜屏障的完整性.

abstractsObjective To study the expression of occludin and zonula occludens-1 (ZO-1) in patients with chronic schistosomiasis and its clinical significance.Methods Colonic mucosa specimens of 50 patients with chronic schistosomiasis and 9 cases of normal people(as a control group) were selected and the expressions of occludin and ZO-1 in colonic epithelial cells were determined using immunohistochemical staining(SABC).Results Compared with the normal control group,the structure of colonic gland was incomplete with glandular atrophy,mean gray values(159.26 ± 6.57,166.07 ± 5.44) of the positive particles of occludin and ZO-1 of colonic epithelial cells in the chronic schistosomiasis group were significantly higher than those of the normal control group(155.53 ± 5.90,162.16 ± 5.14,P ＜ 0.05),but positive unit values(37.27 ± 2.63,34.69 ± 2.19) were significantly lower than those of the control group(38.85 ± 2.42,36.37 ± 2.11,P ＜ 0.05); reduced expression of occludin and ZO-1 in colonic gland of the patients with chronic schistosomiasis had nothing to do with sex or age (P ＞ 0.05).Conclusions Infection of schistosome can reduce the expression of occludin and ZO-1 which can damage the tight junction between colonic epithelium cells,thereby undermining the integrity of the colonic mucosa barrier.