摘要目的 探讨亚慢性砷中毒对大鼠精子透明质酸酶活性及活性强度的影响.方法 清洁级SD雄性大鼠40只,体质量100～120 g,按体质量随机分为对照组、低剂量组、中剂量组和高剂量组4组,每组10只,分笼喂养.低、中、高剂量组大鼠自由饮用含三氧化二砷(As2O3)的蒸馏水,含砷量分别是25、50和100mg/L.每周称重1次,对照组自由饮蒸馏水,连续喂养3个月.实验结束后处死大鼠,取附睾精子,采用改良固定底物膜法检测各组大鼠精子透明质酸酶阳性率及活性强度.结果 对照组,低、中、高剂量组精子顶体内透明质酸酶的阳性率分别为(90.8±13.2)％、(79.1±15.6)％、(59.7±20.3)％、(33.8±14.5)％,中、高剂量组均低于对照组(P均＜ 0.05).对照组、低、中、高剂量组精子顶体内透明质酸酶的活性强度分别为:(45.1±24.0)、(31.1±21.3)、(19.4±15.3)、(9.8±3.1)μm,中、高剂量组均低于对照组(P均＜0.05).结论 亚慢性砷中毒导致精子顶体内透明质酸酶的阳性率及活性强度降低,从而导致精子的受精能力降低.

abstractsObjective To investigate the effects of sub-chronic arsenic poisoning on hyaluronidase activity of rat sperm.Methods Forty Sprague-Dawley (SD) rats in cleanliness grade,weighing 100-200 g,were randomly divided into four groups:control group,low-dose,middle-dose and high-dose arsenic exposure groups,10 in each group,and allotted to cages.Rats of arsenic groups were exposed to arsenic through drinking water (As2O3 was dissolved in distilled water).The concentrations of As2O3 in low-dose,middle-dose and high-dose groups were 25,50,and 100 mg/L,respectively.Body weight of each rat was weighed once a week.The rats in control group drank distilled water freely.All rats were fed for three months.After the experiment,all rats were killed and their sperms were collected.The positive rates and hyaluronidase activity intensities of the sperms in different groups were examined by the mature improved fixed-substrate film method.Results The positive rate of sperm hyaluronidase in control,low-dose,middle-dose and high-dose groups were (90.8 ± 13.2)％,(79.1 ± 15.6)％,(59.7 ± 20.3)％ and (33.8 ± 14.5)％,respectively.Compared with the control group,the positive rates of the middledose and the high-dose groups were significantly reduced(all P ＜ 0.05).The activity intensities of hyaluronidase in the sperms in control,low-dose,middle-dose and high-dose groups were (45.1 ± 24.0),(31.1 ± 21.3),(19.4 ± 15.3) and (9.8 ± 3.1)μm,respectively.Compared with the control group,the activity intensities of hyaluronidase of the middle-dose and the high-dose groups were significantly reduced(all P ＜ 0.05).Conclusion Sub-chronic arsenic poisoning reduces the activity intensities and positive rates of sperm hyaluronidase of rat,resulting in reduced sperm fertilizing capacity.