摘要目的：观察不同剂量亚砷酸钠(NaAsO2）对PC12细胞(具有神经元特性的大鼠嗜铬瘤细胞）色素上皮衍生因子(PEDF）和凋亡相关因子mRNA表达的影响。方法选择PC12细胞作为研究对象,给予不同剂量的NaAsO2[0(对照组）、2、5、10μmol/L]染毒24 h,使用Real-time荧光定量PCR检测不同剂量NaAsO2染毒后PC12细胞的PEDF及其凋亡相关因子Bax和Bcl-2 mRNA的表达水平。结果 PEDF mRNA表达组间比较差异有统计学意义(F=102.28,P<0.05）,其中2、5、10μmol/L NaAsO2染毒组的PEDF mRNA表达(0.70±0.07、0.33±0.04、0.23±0.10）均低于对照组(1.15±0.11,P<0.05）。 Bax mRNA表达组间比较差异无统计学意义(0、2、5、10μmol/L组分别为0.95±0.12、0.80±0.11、0.88±0.11、1.01±0.11,F=2.01,P>0.05）；Bcl-2 mRNA表达组间比较差异有统计学意义(F=19.87,P<0.05）,其中2、5、10μmol/L NaAsO2染毒组Bcl-2 mRNA表达(0.65±0.03、0.49±0.04、0.57±0.09）均低于对照组(0.95±0.11,P均<0.05）；Bax/Bcl-2 mRNA表达比值组间比较差异有统计学意义(F=8.35,P<0.05）,其中5、10μmol/L NaAsO2染毒组的Bax/Bcl-2 mRNA表达比值(1.80±0.72、1.82±0.36）高于对照组(1.02±0.24,P均<0.05）。结论 NaAsO2可能通过降低PC12细胞PEDF mRNA的表达来增加凋亡相关因子Bax/Bcl-2 mRNA表达,继而引起细胞凋亡。

abstractsObjective To observe the effects of different levels of sodium arsenite ( NaAsO2) on mRNA expression of pigment epithelium-derived factor (PEDF) and apoptosis-related factors in PC12 cells ( rat neuron properties pheochromocytoma). Methods PC12 cells were treated with different levels of NaAsO2 [0 (control group), 2, 5, 10 μmol/L] for 24 hours. The mRNA expression of PEDF and apoptosis-related factors (Bax, Bcl-2) were detected by real-time quantitative PCR. Results There were significant differences in the mRNA expressions of PEDF between the 4 groups (F=102.28, P<0.05), the mRNA expressions of PEDF in the group of 2, 5, 10μmol/L (0.70 ± 0.07, 0.33 ± 0.04, 0.23 ± 0.10) was lower than that of control group (1.15 ± 0.11, P< 0.05); there were no significant differences in the mRNA expressions of Bax between the 4 groups (0, 2, 5, 10 μmol/L groups: 0.95 ± 0.12, 0.80 ± 0.11, 0.88 ± 0.11, 1.01 ± 0.11, F= 2.01, P> 0.05); there were significant differences in the mRNA expressions of Bcl-2 between the 4 groups (F=19.87, P<0.05), the mRNA expressions of Bcl-2 in the group of 2, 5, 10 μmol/L (0.65 ± 0.03, 0.49 ± 0.04, 0.57 ± 0.09) were lower than that of control group (0.95 ± 0.11, all P<0.05);there were significant differences in the mRNA expressions of Bax/Bcl-2 between the 4 groups (F=8.352, P<0.05), the mRNA expressions of in the group of 5, 10μmol/L (1.80 ± 0.72, 1.82 ± 0.36) were higher than that of control group (1.02 ± 0.24, all P<0.05). Conclusion NaAsO2 may increase the expression of apoptosis-related factorsBax/Bcl-2 mRNA by decreasing the expression of PEDF mRNA in PC12 cells, leading to apoptosis in PC12 cells.