摘要目的 探讨骨骼肌肌卫星细胞在体外融合成肌管的方式和肌管收缩特点.方法 取新生2d的SD大鼠6只,无菌条件下取股四头肌处的肌卫星细胞进行细胞培养和免疫荧光鉴定;倒置显微镜下观察并记录细胞融合方式和收缩特点.结果 经免疫荧光鉴定,肌卫星细胞表达特异性蛋白Pax7,其纯度为90.3%.观察到单细胞间、单细胞与肌管间和肌管与肌管间的细胞融合方式.在无乙酰胆碱、电刺激和其他细胞接触的条件下,存在自主收缩的肌管.结论 肌卫星细胞在体外可经3种方式融合成自主收缩的肌管.

abstractsObjective To investigate the cell-fusion modes and contractive characteristics of myotubes formed by muscle satellite cells in vitro. Methods The muscle satellite cells in quadriceps femoris of six 2-day-old SD rats were isolated, cultured and identified by immunofluorescence; the cell-fusion modes and contractive characteristics of myotubes were observed and recorded under microscope. Results The positve cells expressing marker Pax7 were obtained by immunofluorescence, and the purity was 90.3%. Myotubes were fused by several cells, cell and myotube or myotube and myotube. There were myotubes of spontaneous contraction on the conditions of no acetylcholine, no electric stimulation or no contiguous cells. Conclusion The myotubes of spontaneous contraction are fused by muscle satellite cells through three cell-fusion modes in vitro.