摘要目的 了解呼和浩特地区分离人源布鲁菌的分子流行病学特征,为指导布鲁菌感染防治提供实验依据.方法 对2013-2015年内蒙古医科大学附属医院分离的27株人源布鲁菌进行常规细菌学和分子鉴定;采用多位点可变数目串联重复序列分析(MLVA)-16方法,对其中9株菌进行分子分型及聚类分析;采用PCR方法对16个毒力基因在27株菌株中的分布情况进行检测和分析.结果 27株菌常规细菌学方法和PCR方法均鉴定为羊种布鲁菌,其中有6株为羊种1型,21株为羊种3型.MLVA-16分型结果显示,9株菌在部分VNTR位点上存在重复数目差异.聚类分析显示,9株菌呈现7个基因型,提示为零星散发病例;同时有2株菌共享同一基因型,提示存在流行病学关联,可能来自同一感染来源.16个毒力基因在27株菌株中均能检测到.结论 呼和浩特地区分离人源布鲁菌主要为羊种1型和3型,毒力基因分布特征相近;部分克隆株存在流行趋势,流行机制值得进一步探究.

abstractsObjective To get knowledge of the molecular epidemiological characteristics of human derived Brucella isolated in Hohhot,and to provide experimental basis in guiding prevention and treatment of Brucella infection.Methods Twenty-seven Brucella isolates derived from patients in Affiliated Hospital of Inner Mongolian Medical University from 2013 to 2015 were identified by routine bacteriological methods and molecular methods.Multiple-locus variable number tandem repeats analysis (MLVA-16) was used to detect molecular typing and do cluster analysis.Sixteen virulent genes were detected and analyzed by polymerase chain reaction (PCR).Results Twenty-seven Brucella isolates were identified as Brucella melitensis (B.melitensis) by routine bacteriological methods and PCR.Out of them,six isolates were B.melitensis biovar 1,and twenty-one isolates were B.melitensis biovar 3.MLVA-16 analysis showed that seven genotypes were obtained from nine Brucella isolates,which showed significant difference in variable number of tandem repeats,which suggested that they originated from sporadic outbreak.Moreover,two isolates were clustered into the same clade,which suggested they were epidemiologically correlated and may be derived from the same origin.Sixteen virulent genes were detected in all of the twenty-seven isolates.Conclusions Brucella isolates from patients in Hohhot are mainly B.melitensis biovar 3 and B.melitensis biovar 1,and the distribution profile of multiple virulence genes is similar.Some isolates have showed epidemic correlation,and the epidemic mechanism should be further explored.