摘要目的 了解高密市小肠结肠炎耶尔森菌的宿主分布、血清型和毒力基因情况.方法 2012-2015年对在高密市采集的各类家畜家禽粪便、腹泻患者粪便、苍蝇以及冷冻(藏)生熟肉制品标本进行检测,分离到的可疑菌株送至山东省疾病预防控制中心进行系统生化鉴定、血清学分型和毒力基因[小肠结肠炎耶尔森菌黏附侵袭点基因(ail)、耐热性肠毒素基因(ystA)、生物1A型携带的肠毒素基因(ystB)、黏附素(yadA)、yop调节子转录活化作用因子(virF)]检测.结果 共监测各类标本2 860份,分离出85株小肠结肠炎耶尔森菌,总检出率为3.0％(85/2 860);其中鸡源株占50.6％(43/85),猪源株占20.0％(17/85),羊源株占10.6％(9/85);所分离菌株的主要血清型是O∶8,占24.7％(21/85),其次是O∶5,占12.9％(11/85).从鸡、鸭、猪等家畜家禽粪便,腹泻患者粪便以及冷冻(藏)生熟肉制品标本均检出小肠结肠炎耶尔森菌,生物分型为1A型;从猪粪便中分离到1株致病性O∶3/4型小肠结肠炎耶尔森菌,携带有ail、ystA、yadA和virF 4种毒力基因.结论 高密市小肠结肠炎耶尔森菌宿主在家畜、家禽中分布较为广泛,致病性和非致病性菌株同时存在,主要以血清型O:5/1A、O:8/1A为主,流行的致病性血清型为O∶3/4型.鸡、猪、羊是主要的动物宿主.

abstractsObjective To investigate the distribution of host animals and the serological distribution and virulence gene of Yersinia eterocolitica in Gaomi City.Methods Sample stools of poultry and domestic and diarrhea patients,as well as flies and chilled (frozen) poultry and domestic animal meats in Gaomi City were preliminarily identified and the suspected strains isolated were sent to Shandong Provincial Center for Disease Control and Prevention for further identification from 2012 to 2015.The bacteria were analyzed by the systematic biochemistry to determine the serotypes,bio-types and virulence genes [Yersinia enterocolitica adhesion aggression site gene (ail),heat-tolerance enterotoxin gene (ystA),organism type 1A enterotoxin gene (ystB),adhesin (yadA),yop regulator transcription activation action factor (virF)].Results Eighty-five stains of Yersinia enterocolitica were detected in 2 860 specimens and the detection rate was 3.0％ (85/2 860).The chicken strains accounted for 50.6％ (43/85),the swine strains accounted for 20.0％ (17/85),and the sheep strains accounted for 10.6％ (9/85).In all the serotypes,the highest was O ∶ 8 (24.7％,21/85),followed by O ∶ 5 (12.9％,11/85),the bacteria were detected in the stools of chickens,duck,and swine etc.,as well as in diarrhea patients and chilled (frozen) poultry and domestic animal meats,biotype is 1A.One strain O ∶ 3/4 was detected from swine stool with ail,ystA,yadA and virF genes.Conclusions The Yersinia enterocolitica is widely distributed and both pathogenic and non-pathogenic strains coexist in Gaomi City;the main serological types are O ∶ 5/1A and O ∶ 8/1A;the pathogenic serotype is O ∶ 3/4,and chicken,swine and sheep are the main host animals.