摘要目的 调查青海省藏系绵羊鼠疫血清流行病学特征,了解青海省藏系绵羊鼠疫流行情况.方法 2013-2016年在青海省8个州(市)采集藏羊颈静脉全血12710份,分离血清后采用鼠疫间接血凝试验(IHA)、胶体金纸上色谱(GICA)两种方法进行鼠疫F1抗体血清学检测,分析鼠疫在青海省各地的流行态势.结果 两种方法均检出鼠疫F1抗体阳性血清86份,阳性检出率为0.68％(86/12710),其中海南州兴海县阳性检出率最高,为5.20％(27/519).海西州、玉树州为藏系绵羊鼠疫历史疫区,阳性检出率分别为0.65％(15/2313)、0.26％(6/2293);海南州、果洛州、黄南州为藏系绵羊鼠疫流行新疫区,阳性检出率分别为1.61％(28/1741)、1.01％(15/1481)、1.44％(19/1316).鼠疫血清抗体滴度介于1:20～1:5120,其中果洛州玛沁县抗体滴度最高,为1:5120.结论 藏系绵羊鼠疫在青海省流行形式为散发,局部地区存在爆发流行.应加强藏系绵羊鼠疫的监测工作,尤其是在旱獭鼠疫流行区,更不能忽略.

abstractsObjective In order to acquaint with the prevalence of Tibetan sheep plague in this area, we conducted a serum epidemiological investigation of Tibetan sheep plague in Qinghai Province. Methods Indirect hemagglutination assay (IHA) and colloidal gold immunochromatography (GICA) were applied to test serum samples of Tibetan sheep and whole blood samples from jugular vein of Tibetan sheep were collected in 8 Prefectures of Qinghai Province from 2013 to 2016. Results A total of 86 positive Tibetan sheep serum samples with plague F1 antibody were detected by both methods, and the positive rate was 0.68％ (86/12710), the samples collected in Xinghai County Hainan Prefecture had the highest positive rate, which was 5.20％ (27/519). The Haixi Prefecture and Yushu Prefecture were historical epidemic areas, the positive rates were 0.65％(15/2313) and 0.26％(6/2293), respectively. Hainan Prefecture, Guoluo Prefacture and Huangnan Prefecture were newly confirmed epidemic areas, the positive rates were 1.61％ (28/1741), 1.01％ (15/1481), and 1.44％(19/1316), respectively. The antibody titers were 1:20 to 1:5120, the samples collected in Maqin County Guoluo Prefecture had the highest titer, namely 1 :5120. Conclusions In Qinghai Province, Tibetan sheep plague is endemic, and there are outbreaks in some regions. So we have to enhance the Tibetan sheep plague monitoring especially in Marmot plague epidemic area.