摘要目的 验证国家卫生标准研制项目血清中碘的测定电感耦合等离子体质谱测定方法.方法 采用稀释剂稀释血清样品和标准系列的前处理方法,应用电感耦合等离子体质谱(ICP-MS)仪直接测定碘含量,从标准曲线的相关系数、检出限、精密度、准确度等方面对此方法进行测试实验.结果 血清碘浓度范围为0 ～ 300μg/L时,与仪器测定的碘离子计数的线性相关系数为0.999 8～1.000 0(n=6),血清碘最低检出限为1.24μg/L.7份不同血清样测定的批内和批间精密度实验的相对标准偏差(RSD)范围分别为0.5％～1.5％和0.3％～1.4％;对含碘量40～ 230 μg/L的7个血清样加标回收实验的回收率范围为93.4％～109.2％,总平均回收率为102.4％.结论 血清样品无需进行消化前处理,经稀释剂稀释后可直接进样,无需使用剧毒物质,实现检测过程自动化,灵敏度和精密度高、准确性好,适合血清碘含量的快速定量测定.

abstractsObjective To determine serum iodine by inductively coupled plasma mass spectrometry (ICP-MS).Methods ICP-MS was used to determine iodine content directly by pretreatment method of diluting serum samples and standard series with diluent.The method was tested from correlation coefficient of standard curve,detection limit,precision,accuracy,etc.Results When the concentration of iodine in serum ranged from 0 to 300 μg/L,the linear correlation coefficient with the iodine ion counts determined by the instrument was 0.999 8 to 1.000 0 (n =6);the detection limit of iodine in serum was 1.24 μg/L;the relative standard deviation (RSD) ranged from 0.5％ to 1.5％ and from 0.3％ to 1.4％ for the intra-and inter-batch precision tests of seven different serum samples,respectively;the recovery rate of 7 serum samples with iodine content 40-230 μg/L ranged from 93.4％ to 109.2％,and the total average recovery rate was 102.4％.Conclusions Serum samples need not be pretreated before digestion,and can be directly injected after dilution with diluent without using highly toxic substances.The detection process is automatic,sensitive,precise,accurate and suitable for rapid quantitative determination of serum iodine content.